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Enzymatic transformation of ginsenosides in Korean Red Ginseng (Panax ginseng Meyer) extract prepared by Spezyme and Optidex

BACKGROUND: In this study, we examined the effects of various enzymes on chemical conversions of ginsenosides in ginseng extract prepared by amylases. METHODS: Rapidase, Econase CE, Viscozyme, Ultraflo L, and Cytolase PCL5 were used for secondary enzymatic hydrolysis after amylase treatment of ginse...

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Autores principales: Choi, Hyeon-Son, Kim, Sun Young, Park, Yooheon, Jung, Eun Young, Suh, Hyung Joo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4213822/
https://www.ncbi.nlm.nih.gov/pubmed/25379006
http://dx.doi.org/10.1016/j.jgr.2014.05.005
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author Choi, Hyeon-Son
Kim, Sun Young
Park, Yooheon
Jung, Eun Young
Suh, Hyung Joo
author_facet Choi, Hyeon-Son
Kim, Sun Young
Park, Yooheon
Jung, Eun Young
Suh, Hyung Joo
author_sort Choi, Hyeon-Son
collection PubMed
description BACKGROUND: In this study, we examined the effects of various enzymes on chemical conversions of ginsenosides in ginseng extract prepared by amylases. METHODS: Rapidase, Econase CE, Viscozyme, Ultraflo L, and Cytolase PCL5 were used for secondary enzymatic hydrolysis after amylase treatment of ginseng extract, and ginsenoside contents, skin permeability, and chemical compositions including total sugar, acidic polysaccharide, and polyphenols were determined on the hydrolyzed ginseng extract. RESULTS: Rapidase treatment significantly elevated total ginsenoside contents compared with the control (p < 0.05). In particular, deglycosylated ginsenosides including Rg3, which are known as bioactive compounds, were significantly increased after Rapidase treatment (p < 0.05). The Rapidase-treated group also increased the skin permeability of polyphenols compared with the control, showing the highest level of total sugar content among the enzyme treatment groups. CONCLUSION: This result showed that Rapidase induced the conversion of ginsenoside glycosides to aglycones. Meanwhile, Cytolase PCL5 and Econase treatments led to a significant increase of uronic acid (acidic polysaccharide) level. Taken together, our data showed that the treatments of enzymes including Rapidase are useful for the conversion and increase of ginsenosides in ginseng extracts or products.
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spelling pubmed-42138222014-11-06 Enzymatic transformation of ginsenosides in Korean Red Ginseng (Panax ginseng Meyer) extract prepared by Spezyme and Optidex Choi, Hyeon-Son Kim, Sun Young Park, Yooheon Jung, Eun Young Suh, Hyung Joo J Ginseng Res Research Article BACKGROUND: In this study, we examined the effects of various enzymes on chemical conversions of ginsenosides in ginseng extract prepared by amylases. METHODS: Rapidase, Econase CE, Viscozyme, Ultraflo L, and Cytolase PCL5 were used for secondary enzymatic hydrolysis after amylase treatment of ginseng extract, and ginsenoside contents, skin permeability, and chemical compositions including total sugar, acidic polysaccharide, and polyphenols were determined on the hydrolyzed ginseng extract. RESULTS: Rapidase treatment significantly elevated total ginsenoside contents compared with the control (p < 0.05). In particular, deglycosylated ginsenosides including Rg3, which are known as bioactive compounds, were significantly increased after Rapidase treatment (p < 0.05). The Rapidase-treated group also increased the skin permeability of polyphenols compared with the control, showing the highest level of total sugar content among the enzyme treatment groups. CONCLUSION: This result showed that Rapidase induced the conversion of ginsenoside glycosides to aglycones. Meanwhile, Cytolase PCL5 and Econase treatments led to a significant increase of uronic acid (acidic polysaccharide) level. Taken together, our data showed that the treatments of enzymes including Rapidase are useful for the conversion and increase of ginsenosides in ginseng extracts or products. 2014-05-23 2014-10-15 /pmc/articles/PMC4213822/ /pubmed/25379006 http://dx.doi.org/10.1016/j.jgr.2014.05.005 Text en © 2014 The Korean Society of Ginseng. Published by Elsevier B.V. All rights reserved. http://creativecommons.org/licenses/by-nc/3.0/ This is an Open Access article distributed under the terms of the CC-BY-NC License (http://creativecommons.org/licenses/by-nc/3.0).
spellingShingle Research Article
Choi, Hyeon-Son
Kim, Sun Young
Park, Yooheon
Jung, Eun Young
Suh, Hyung Joo
Enzymatic transformation of ginsenosides in Korean Red Ginseng (Panax ginseng Meyer) extract prepared by Spezyme and Optidex
title Enzymatic transformation of ginsenosides in Korean Red Ginseng (Panax ginseng Meyer) extract prepared by Spezyme and Optidex
title_full Enzymatic transformation of ginsenosides in Korean Red Ginseng (Panax ginseng Meyer) extract prepared by Spezyme and Optidex
title_fullStr Enzymatic transformation of ginsenosides in Korean Red Ginseng (Panax ginseng Meyer) extract prepared by Spezyme and Optidex
title_full_unstemmed Enzymatic transformation of ginsenosides in Korean Red Ginseng (Panax ginseng Meyer) extract prepared by Spezyme and Optidex
title_short Enzymatic transformation of ginsenosides in Korean Red Ginseng (Panax ginseng Meyer) extract prepared by Spezyme and Optidex
title_sort enzymatic transformation of ginsenosides in korean red ginseng (panax ginseng meyer) extract prepared by spezyme and optidex
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4213822/
https://www.ncbi.nlm.nih.gov/pubmed/25379006
http://dx.doi.org/10.1016/j.jgr.2014.05.005
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