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Impairment of Hepatic and Renal Functions by 2,5-Hexanedione Is Accompanied by Oxidative Stress in Rats

2,5-Hexanedione (2,5-HD) is the toxic metabolite of n-hexane which is widely used as solvent in numerous industries. The present study elucidated the precise mechanism of 2,5-HD in hepatorenal toxicity by determining the involvement of oxidative stress in rats. Adult male Wistar rats were exposed to...

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Autores principales: Adedara, Isaac A., Abolaji, Amos O., Odion, Blessing E., Okwudi, Isioma J., Omoloja, Abiola A., Farombi, Ebenezer O.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4214033/
https://www.ncbi.nlm.nih.gov/pubmed/25379039
http://dx.doi.org/10.1155/2014/239240
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author Adedara, Isaac A.
Abolaji, Amos O.
Odion, Blessing E.
Okwudi, Isioma J.
Omoloja, Abiola A.
Farombi, Ebenezer O.
author_facet Adedara, Isaac A.
Abolaji, Amos O.
Odion, Blessing E.
Okwudi, Isioma J.
Omoloja, Abiola A.
Farombi, Ebenezer O.
author_sort Adedara, Isaac A.
collection PubMed
description 2,5-Hexanedione (2,5-HD) is the toxic metabolite of n-hexane which is widely used as solvent in numerous industries. The present study elucidated the precise mechanism of 2,5-HD in hepatorenal toxicity by determining the involvement of oxidative stress in rats. Adult male Wistar rats were exposed to 0, 0.25, 0.5, and 1% 2,5-HD in drinking water for 21 days. Exposure to 2,5-HD caused liver and kidney atrophy evidenced by significant elevation in serum aminotransferases, alkaline phosphatase, albumin, bilirubin, urea, creatinine, and electrolytes levels compared with control. The marked dose-dependent increase in total cholesterol (TC), triglyceride (TG), and low-density lipoprotein (LDL) was accompanied with significant decrease in high-density lipoprotein (HDL) levels in 2,5-HD-exposed animals when compared with the control. Administration of 2,5-HD significantly diminished glutathione (GSH) level but increased the activities of superoxide dismutase (SOD), catalase, glutathione peroxidase (GPx), and glutathione-S-transferase (GST) concomitantly with marked elevation in hydrogen peroxide (H(2)O(2)) and malondialdehyde (MDA) levels in liver and kidney of the treated groups compared with control. These findings suggest that undue exposure to 2,5-HD at environmentally relevant levels may impair liver and kidney functions through induction of oxidative stress.
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spelling pubmed-42140332014-11-06 Impairment of Hepatic and Renal Functions by 2,5-Hexanedione Is Accompanied by Oxidative Stress in Rats Adedara, Isaac A. Abolaji, Amos O. Odion, Blessing E. Okwudi, Isioma J. Omoloja, Abiola A. Farombi, Ebenezer O. J Toxicol Research Article 2,5-Hexanedione (2,5-HD) is the toxic metabolite of n-hexane which is widely used as solvent in numerous industries. The present study elucidated the precise mechanism of 2,5-HD in hepatorenal toxicity by determining the involvement of oxidative stress in rats. Adult male Wistar rats were exposed to 0, 0.25, 0.5, and 1% 2,5-HD in drinking water for 21 days. Exposure to 2,5-HD caused liver and kidney atrophy evidenced by significant elevation in serum aminotransferases, alkaline phosphatase, albumin, bilirubin, urea, creatinine, and electrolytes levels compared with control. The marked dose-dependent increase in total cholesterol (TC), triglyceride (TG), and low-density lipoprotein (LDL) was accompanied with significant decrease in high-density lipoprotein (HDL) levels in 2,5-HD-exposed animals when compared with the control. Administration of 2,5-HD significantly diminished glutathione (GSH) level but increased the activities of superoxide dismutase (SOD), catalase, glutathione peroxidase (GPx), and glutathione-S-transferase (GST) concomitantly with marked elevation in hydrogen peroxide (H(2)O(2)) and malondialdehyde (MDA) levels in liver and kidney of the treated groups compared with control. These findings suggest that undue exposure to 2,5-HD at environmentally relevant levels may impair liver and kidney functions through induction of oxidative stress. Hindawi Publishing Corporation 2014 2014-10-15 /pmc/articles/PMC4214033/ /pubmed/25379039 http://dx.doi.org/10.1155/2014/239240 Text en Copyright © 2014 Isaac A. Adedara et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Adedara, Isaac A.
Abolaji, Amos O.
Odion, Blessing E.
Okwudi, Isioma J.
Omoloja, Abiola A.
Farombi, Ebenezer O.
Impairment of Hepatic and Renal Functions by 2,5-Hexanedione Is Accompanied by Oxidative Stress in Rats
title Impairment of Hepatic and Renal Functions by 2,5-Hexanedione Is Accompanied by Oxidative Stress in Rats
title_full Impairment of Hepatic and Renal Functions by 2,5-Hexanedione Is Accompanied by Oxidative Stress in Rats
title_fullStr Impairment of Hepatic and Renal Functions by 2,5-Hexanedione Is Accompanied by Oxidative Stress in Rats
title_full_unstemmed Impairment of Hepatic and Renal Functions by 2,5-Hexanedione Is Accompanied by Oxidative Stress in Rats
title_short Impairment of Hepatic and Renal Functions by 2,5-Hexanedione Is Accompanied by Oxidative Stress in Rats
title_sort impairment of hepatic and renal functions by 2,5-hexanedione is accompanied by oxidative stress in rats
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4214033/
https://www.ncbi.nlm.nih.gov/pubmed/25379039
http://dx.doi.org/10.1155/2014/239240
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