Genome-Wide Distribution of RNA-DNA Hybrids Identifies RNase H Targets in tRNA Genes, Retrotransposons and Mitochondria

During transcription, the nascent RNA can invade the DNA template, forming extended RNA-DNA duplexes (R-loops). Here we employ ChIP-seq in strains expressing or lacking RNase H to map targets of RNase H activity throughout the budding yeast genome. In wild-type strains, R-loops were readily detected...

Descripción completa

Detalles Bibliográficos
Autores principales: El Hage, Aziz, Webb, Shaun, Kerr, Alastair, Tollervey, David
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2014
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4214602/
https://www.ncbi.nlm.nih.gov/pubmed/25357144
http://dx.doi.org/10.1371/journal.pgen.1004716

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4214602/
https://www.ncbi.nlm.nih.gov/pubmed/25357144
http://dx.doi.org/10.1371/journal.pgen.1004716

Ejemplares similares