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Some personal and historical notes on the utility of “deep-etch” electron microscopy for making cell structure/function correlations
This brief essay talks up the advantages of metal replicas for electron microscopy and explains why they are still the best way to image frozen cells in the electron microscope. Then it explains our approach to freezing, namely the Van Harreveld trick of “slamming” living cells onto a supercold bloc...
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
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The American Society for Cell Biology
2014
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4214773/ https://www.ncbi.nlm.nih.gov/pubmed/25360049 http://dx.doi.org/10.1091/mbc.E14-05-1016 |