Cargando…

DNA polymerases engineered by directed evolution to incorporate non-standard nucleotides

DNA polymerases have evolved for billions of years to accept natural nucleoside triphosphate substrates with high fidelity and to exclude closely related structures, such as the analogous ribonucleoside triphosphates. However, polymerases that can accept unnatural nucleoside triphosphates are desire...

Descripción completa

Detalles Bibliográficos
Autores principales:	Laos, Roberto, Thomson, J. Michael, Benner, Steven A.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Frontiers Media S.A. 2014
Materias:	Microbiology
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4215692/ https://www.ncbi.nlm.nih.gov/pubmed/25400626 http://dx.doi.org/10.3389/fmicb.2014.00565

Ejemplares similares

Snapshots of an evolved DNA polymerase pre- and post-incorporation of an unnatural nucleotide
por: Singh, Isha, et al.
Publicado: (2018)

PCNA accelerates the nucleotide incorporation rate by DNA polymerase δ
por: Mondol, Tanumoy, et al.
Publicado: (2019)

INCORPORATION OF NUCLEOTIDES INTO NUCLEI OF FIXED CELLS BY DNA POLYMERASE
por: von Borstel, R. C., et al.
Publicado: (1966)

A Model for the Evolution of Nucleotide Polymerase Directionality
por: Ballanco, Joshua, et al.
Publicado: (2011)

Engineered split in Pfu DNA polymerase fingers domain improves incorporation of nucleotide γ-phosphate derivative
por: Hansen, Connie J., et al.
Publicado: (2011)

Measurement of incorporation kinetics of non-fluorescent native nucleotides by DNA polymerases using fluorescence microscopy
por: Walsh, Matthew T., et al.
Publicado: (2017)

DNA polymerase θ specializes in incorporating synthetic expanded-size (xDNA) nucleotides
por: Kent, Tatiana, et al.
Publicado: (2016)

Engineering processive DNA polymerases with maximum benefit at minimum cost
por: Reha-Krantz, Linda J., et al.
Publicado: (2014)

Fluorinated oil-surfactant mixtures with the density of water: Artificial cells for synthetic biology
por: Laos, Roberto, et al.
Publicado: (2022)

Evolution of replicative DNA polymerases in archaea and their contributions to the eukaryotic replication machinery
por: Makarova, Kira S., et al.
Publicado: (2014)

NETSeq reveals heterogeneous nucleotide incorporation by RNA polymerase I
por: Clarke, Andrew M., et al.
Publicado: (2018)

DNA polymerases in biotechnology
por: Gardner, Andrew F., et al.
Publicado: (2014)

Mutant Taq DNA polymerases with improved elongation ability as a useful reagent for genetic engineering
por: Yamagami, Takeshi, et al.
Publicado: (2014)

Directed adenovirus evolution using engineered mutator viral polymerases
por: Uil, Taco G., et al.
Publicado: (2011)

Critical amino acids in human DNA polymerases η and κ involved in erroneous incorporation of oxidized nucleotides
por: Katafuchi, Atsushi, et al.
Publicado: (2010)

Structural and kinetic insights into binding and incorporation of L-nucleotide analogs by a Y-family DNA polymerase
por: Gaur, Vineet, et al.
Publicado: (2014)

Protocol for monitoring and analyzing single nucleotide incorporation by S. cerevisiae RNA polymerases
por: Jacobs, Ruth Q., et al.
Publicado: (2023)

The Pseudorabies Virus DNA Polymerase Accessory Subunit UL42 Directs Nuclear Transport of the Holoenzyme
por: Wang, Yi-Ping, et al.
Publicado: (2016)

Incorporation of inosine into DNA by human polymerase eta (Polη): kinetics of nucleotide misincorporation and structural basis for the mutagenicity
por: Zhang, Qi, et al.
Publicado: (2023)

Directed evolution engineering to improve activity of glucose dehydrogenase by increasing pocket hydrophobicity
por: Hua, Li, et al.
Publicado: (2022)

Bacteriophage T7 DNA polymerase – sequenase
por: Zhu, Bin
Publicado: (2014)

Enhanced Production of Pterostilbene in Escherichia coli Through Directed Evolution and Host Strain Engineering
por: Yan, Zhi-Bo, et al.
Publicado: (2021)

Synthetic Nucleotides as Probes of DNA Polymerase Specificity
por: Walsh, Jason M., et al.
Publicado: (2012)

Significant contribution of the 3′→5′ exonuclease activity to the high fidelity of nucleotide incorporation catalyzed by human DNA polymerase ϵ
por: Zahurancik, Walter J., et al.
Publicado: (2014)

Building better polymerases: Engineering the replication of expanded genetic alphabets
por: Ouaray, Zahra, et al.
Publicado: (2020)

Calicivirus RNA-Dependent RNA Polymerases: Evolution, Structure, Protein Dynamics, and Function
por: Smertina, Elena, et al.
Publicado: (2019)

Expanding the repertoire of DNA polymerase substrates: template-instructed incorporation of non-nucleoside triphosphate analogues by DNA polymerases β and λ
por: Crespan, Emmanuele, et al.
Publicado: (2007)

Engineering of a DNA Polymerase for Direct m(6)A Sequencing
por: Aschenbrenner, Joos, et al.
Publicado: (2017)

Direct Enzyme Engineering of B Family DNA Polymerases for Biotechnological Approaches
por: Kuznetsova, Aleksandra A., et al.
Publicado: (2023)

Nucleotide binding interactions modulate dNTP selectivity and facilitate 8-oxo-dGTP incorporation by DNA polymerase lambda
por: Burak, Matthew J., et al.
Publicado: (2015)

Antibody–nucleotide conjugate as a substrate for DNA polymerases
por: Balintová, J., et al.
Publicado: (2018)

Therminator DNA Polymerase: Modified Nucleotides and Unnatural Substrates
por: Gardner, Andrew F., et al.
Publicado: (2019)

DNA polymerases drive DNA sequencing-by-synthesis technologies: both past and present
por: Chen, Cheng-Yao
Publicado: (2014)

Incorporating the Detection of Single Nucleotide Polymorphisms Associated With Invasive Aspergillosis Into the Clinic
por: White, P. Lewis, et al.
Publicado: (2022)

Increased spontaneous recombination in RNase H2-deficient cells arises from multiple contiguous rNMPs and not from single rNMP residues incorporated by DNA polymerase epsilon
por: Epshtein, Anastasiya, et al.
Publicado: (2016)

Characterization of the Elongation Complex of Dengue Virus RNA Polymerase: ASSEMBLY, KINETICS OF NUCLEOTIDE INCORPORATION, AND FIDELITY
por: Jin, Zhinan, et al.
Publicado: (2011)

A Bacteriophage DNA Mimic Protein Employs a Non-specific Strategy to Inhibit the Bacterial RNA Polymerase
por: Wang, Zhihao, et al.
Publicado: (2021)

PCR performance of a thermostable heterodimeric archaeal DNA polymerase
por: Killelea, Tom, et al.
Publicado: (2014)

Catalytic properties of RNA polymerases IV and V: accuracy, nucleotide incorporation and rNTP/dNTP discrimination
por: Marasco, Michelle, et al.
Publicado: (2017)

A polar filter in DNA polymerases prevents ribonucleotide incorporation
por: Johnson, Mary K, et al.
Publicado: (2019)

Cannot write session to /tmp/vufind_sessions/sess_73j2ormqcc58p3ok69v21jv2lr