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High-resolution mapping of transcriptional dynamics across tissue development reveals a stable mRNA–tRNA interface

The genetic code is an abstraction of how mRNA codons and tRNA anticodons molecularly interact during protein synthesis; the stability and regulation of this interaction remains largely unexplored. Here, we characterized the expression of mRNA and tRNA genes quantitatively at multiple time points in...

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Autores principales: Schmitt, Bianca M., Rudolph, Konrad L.M., Karagianni, Panagiota, Fonseca, Nuno A., White, Robert J., Talianidis, Iannis, Odom, Duncan T., Marioni, John C., Kutter, Claudia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cold Spring Harbor Laboratory Press 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4216921/
https://www.ncbi.nlm.nih.gov/pubmed/25122613
http://dx.doi.org/10.1101/gr.176784.114
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author Schmitt, Bianca M.
Rudolph, Konrad L.M.
Karagianni, Panagiota
Fonseca, Nuno A.
White, Robert J.
Talianidis, Iannis
Odom, Duncan T.
Marioni, John C.
Kutter, Claudia
author_facet Schmitt, Bianca M.
Rudolph, Konrad L.M.
Karagianni, Panagiota
Fonseca, Nuno A.
White, Robert J.
Talianidis, Iannis
Odom, Duncan T.
Marioni, John C.
Kutter, Claudia
author_sort Schmitt, Bianca M.
collection PubMed
description The genetic code is an abstraction of how mRNA codons and tRNA anticodons molecularly interact during protein synthesis; the stability and regulation of this interaction remains largely unexplored. Here, we characterized the expression of mRNA and tRNA genes quantitatively at multiple time points in two developing mouse tissues. We discovered that mRNA codon pools are highly stable over development and simply reflect the genomic background; in contrast, precise regulation of tRNA gene families is required to create the corresponding tRNA transcriptomes. The dynamic regulation of tRNA genes during development is controlled in order to generate an anticodon pool that closely corresponds to messenger RNAs. Thus, across development, the pools of mRNA codons and tRNA anticodons are invariant and highly correlated, revealing a stable molecular interaction interlocking transcription and translation.
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spelling pubmed-42169212014-11-04 High-resolution mapping of transcriptional dynamics across tissue development reveals a stable mRNA–tRNA interface Schmitt, Bianca M. Rudolph, Konrad L.M. Karagianni, Panagiota Fonseca, Nuno A. White, Robert J. Talianidis, Iannis Odom, Duncan T. Marioni, John C. Kutter, Claudia Genome Res Research The genetic code is an abstraction of how mRNA codons and tRNA anticodons molecularly interact during protein synthesis; the stability and regulation of this interaction remains largely unexplored. Here, we characterized the expression of mRNA and tRNA genes quantitatively at multiple time points in two developing mouse tissues. We discovered that mRNA codon pools are highly stable over development and simply reflect the genomic background; in contrast, precise regulation of tRNA gene families is required to create the corresponding tRNA transcriptomes. The dynamic regulation of tRNA genes during development is controlled in order to generate an anticodon pool that closely corresponds to messenger RNAs. Thus, across development, the pools of mRNA codons and tRNA anticodons are invariant and highly correlated, revealing a stable molecular interaction interlocking transcription and translation. Cold Spring Harbor Laboratory Press 2014-11 /pmc/articles/PMC4216921/ /pubmed/25122613 http://dx.doi.org/10.1101/gr.176784.114 Text en © 2014 Schmitt et al.; Published by Cold Spring Harbor Laboratory Press http://creativecommons.org/licenses/by/4.0/ This article, published in Genome Research, is available under a Creative Commons License (Attribution 4.0 International), as described at http://creativecommons.org/licenses/by/4.0.
spellingShingle Research
Schmitt, Bianca M.
Rudolph, Konrad L.M.
Karagianni, Panagiota
Fonseca, Nuno A.
White, Robert J.
Talianidis, Iannis
Odom, Duncan T.
Marioni, John C.
Kutter, Claudia
High-resolution mapping of transcriptional dynamics across tissue development reveals a stable mRNA–tRNA interface
title High-resolution mapping of transcriptional dynamics across tissue development reveals a stable mRNA–tRNA interface
title_full High-resolution mapping of transcriptional dynamics across tissue development reveals a stable mRNA–tRNA interface
title_fullStr High-resolution mapping of transcriptional dynamics across tissue development reveals a stable mRNA–tRNA interface
title_full_unstemmed High-resolution mapping of transcriptional dynamics across tissue development reveals a stable mRNA–tRNA interface
title_short High-resolution mapping of transcriptional dynamics across tissue development reveals a stable mRNA–tRNA interface
title_sort high-resolution mapping of transcriptional dynamics across tissue development reveals a stable mrna–trna interface
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4216921/
https://www.ncbi.nlm.nih.gov/pubmed/25122613
http://dx.doi.org/10.1101/gr.176784.114
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