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ROCK Inhibitor Is Not Required for Embryoid Body Formation from Singularized Human Embryonic Stem Cells

We report a technology to form human embryoid bodies (hEBs) from singularized human embryonic stem cells (hESCs) without the use of the p160 rho-associated coiled-coil kinase inhibitor (ROCKi) or centrifugation (spin). hEB formation was tested under four conditions: +ROCKi/+spin, +ROCKi/-spin, -ROCK...

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Detalles Bibliográficos
Autores principales: Pettinato, Giuseppe, Vanden Berg-Foels, Wendy S., Zhang, Ning, Wen, Xuejun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4217711/
https://www.ncbi.nlm.nih.gov/pubmed/25365581
http://dx.doi.org/10.1371/journal.pone.0100742