Cargando…

ROCK Inhibitor Is Not Required for Embryoid Body Formation from Singularized Human Embryonic Stem Cells

We report a technology to form human embryoid bodies (hEBs) from singularized human embryonic stem cells (hESCs) without the use of the p160 rho-associated coiled-coil kinase inhibitor (ROCKi) or centrifugation (spin). hEB formation was tested under four conditions: +ROCKi/+spin, +ROCKi/-spin, -ROCK...

Descripción completa

Detalles Bibliográficos
Autores principales:	Pettinato, Giuseppe, Vanden Berg-Foels, Wendy S., Zhang, Ning, Wen, Xuejun
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Public Library of Science 2014
Materias:	Research Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4217711/ https://www.ncbi.nlm.nih.gov/pubmed/25365581 http://dx.doi.org/10.1371/journal.pone.0100742

Ejemplares similares

Formation of Well-defined Embryoid Bodies from Dissociated Human Induced Pluripotent Stem Cells using Microfabricated Cell-repellent Microwell Arrays
por: Pettinato, Giuseppe, et al.
Publicado: (2014)

Differentiation of Mouse Embryonic Stem Cells into Endoderm without Embryoid Body Formation
por: Kim, Peter T. W., et al.
Publicado: (2010)

Imprinted expression in cystic embryoid bodies shows an embryonic and not an extra-embryonic pattern
por: Kulinski, Tomasz M., et al.
Publicado: (2015)

In vitro differentiation of human embryonic stem cells to hemogenic endothelium and blood progenitors via embryoid body formation
por: Garcia-Alegria, Eva, et al.
Publicado: (2021)

Characterization of tubular liquid crystal structure in embryonic stem cell derived embryoid bodies
por: Xu, MengMeng, et al.
Publicado: (2017)

Formation of a Polarised Primitive Endoderm Layer in Embryoid Bodies Requires Fgfr/Erk Signalling
por: Doughton, Gail, et al.
Publicado: (2014)

TGF-β1 induces the formation of vascular-like structures in embryoid bodies derived from human embryonic stem cells
por: WANG, YAN, et al.
Publicado: (2014)

Transcript profiling and lipidomic analysis of ceramide subspecies in mouse embryonic stem cells and embryoid bodies
por: Park, Hyejung, et al.
Publicado: (2010)

Differential Single Cell Responses of Embryonic Stem Cells Versus Embryoid Bodies to Gravity Mechanostimulation
por: Juran, Cassandra M., et al.
Publicado: (2022)

Microfabric Vessels for Embryoid Body Formation and Rapid Differentiation of Pluripotent Stem Cells
por: Sato, Hiroki, et al.
Publicado: (2016)

Cell Mechanics in Embryoid Bodies
por: Zeevaert, Kira, et al.
Publicado: (2020)

Correction: Formation of a Polarised Primitive Endoderm Layer in Embryoid Bodies Requires Fgfr/Erk Signalling
por: Doughton, Gail, et al.
Publicado: (2015)

Comparison of the gene expression profile of undifferentiated human embryonic stem cell lines and differentiating embryoid bodies
por: Bhattacharya, Bhaskar, et al.
Publicado: (2005)

The Contribution of Y Chromosome Genes to Spontaneous Differentiation of Human Embryonic Stem Cells into Embryoid Bodies In Vitro
por: Nafian Dehkordi, Simin, et al.
Publicado: (2021)

Matrix Rigidity-Modulated Cardiovascular Organoid Formation from Embryoid Bodies
por: Shkumatov, Artem, et al.
Publicado: (2014)

Osteogenic Embryoid Body-Derived Material Induces Bone Formation In Vivo
por: Sutha, Ken, et al.
Publicado: (2015)

Effect of cellular mass on chondrogenic differentiation during embryoid body formation
por: Lach, Michał Stefan, et al.
Publicado: (2018)

ZO-1 and ZO-2 Are Required for Extra-Embryonic Endoderm Integrity, Primitive Ectoderm Survival and Normal Cavitation in Embryoid Bodies Derived from Mouse Embryonic Stem Cells
por: Phua, Dominic C. Y., et al.
Publicado: (2014)

The pro‐inflammatory signature of lipopolysaccharide in spontaneous contracting embryoid bodies differentiated from mouse embryonic stem cells
por: Scharmacher, Jennifer, et al.
Publicado: (2023)

5-Aza Exposure Improves Reprogramming Process Through Embryoid Body Formation in Human Gingival Stem Cells
por: Diomede, Francesca, et al.
Publicado: (2018)

Collagen/β(1) integrin interaction is required for embryoid body formation during cardiogenesis from murine induced pluripotent stem cells
por: Zeng, Di, et al.
Publicado: (2013)

A PDMS-Based Microfluidic Hanging Drop Chip for Embryoid Body Formation
por: Wu, Huei-Wen, et al.
Publicado: (2016)

Cell adhesion and sorting in embryoid bodies derived from N- or E-cadherin deficient murine embryonic stem cells
por: Moore, Robert, et al.
Publicado: (2014)

Development of Mouse Embryonic Stem (ES) Cells: IV Differentiation to Mature T and B Lymphocytes after Implantation of Embryoid Bodies Into Nude Mice
por: Chen, Una, et al.
Publicado: (1995)

Loss of heme oxygenase-1 accelerates mesodermal gene expressions during embryoid body development from mouse embryonic stem cells
por: Lai, Yan-Liang, et al.
Publicado: (2017)

Nox4-IGF2 Axis Promotes Differentiation of Embryoid Body Cells Into Derivatives of the Three Embryonic Germ Layers
por: Kim, Jusong, et al.
Publicado: (2021)

Physical Passaging of Embryoid Bodies Generated from Human Pluripotent Stem Cells
por: Son, Mi-Young, et al.
Publicado: (2011)

The Key Enzyme of the Sialic Acid Metabolism Is Involved in Embryoid Body Formation and Expression of Marker Genes of Germ Layer Formation
por: Weidemann, Wenke, et al.
Publicado: (2013)

From embryos to embryoids: How external signals and self-organization drive embryonic development
por: Morales, J. Serrano, et al.
Publicado: (2021)

Compartmentalized embryoid body culture for induction of spatially patterned differentiation
por: Kaneda, Shohei, et al.
Publicado: (2017)

Hyperthermia Disturbs and Delays Spontaneous Differentiation of Human Embryoid Bodies
por: Kwon, Ji Hyun, et al.
Publicado: (2020)

Lhx2 Expression Promotes Self-Renewal of a Distinct Multipotential Hematopoietic Progenitor Cell in Embryonic Stem Cell-Derived Embryoid Bodies
por: Dahl, Lina, et al.
Publicado: (2008)

Comparison of Germ Cell Gene Expressions in Spontaneous Monolayer versus Embryoid Body Differentiation of Mouse Embryonic Stem Cells toward Germ Cells
por: Gholamitabar Tabari, Maryam, et al.
Publicado: (2019)

Comparison of Cell Viability and Embryoid Body Size of Two Embryonic Stem Cell Lines After Different Exposure Times to Bone Morphogenetic Protein 4
por: Zarei Fard, Nehleh, et al.
Publicado: (2015)

Effects of Simulated Microgravity on Wild Type and Marfan hiPSCs-Derived Embryoid Bodies
por: Spitalieri, Paola, et al.
Publicado: (2021)

Trim33 regulates early maturation of mouse embryoid bodies in vitro
por: Rajderkar, Sudha, et al.
Publicado: (2017)

GSK-J4-Mediated Transcriptomic Alterations in Differentiating Embryoid Bodies
por: Mandal, Chanchal, et al.
Publicado: (2017)

A standardized human embryoid body platform for the detection and analysis of teratogens
por: Flamier, Anthony, et al.
Publicado: (2017)

Adherent but Not Suspension-Cultured Embryoid Bodies Develop into Laminated Retinal Organoids
por: Radojevic, Bojana, et al.
Publicado: (2021)

Resveratrol’s neural protective effects for the injured embryoid body and cerebral organoid
por: Wang, Yanli, et al.
Publicado: (2022)

Cannot write session to /tmp/vufind_sessions/sess_h9pdmp3ce1fsdoe6b4fuk0g97u