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Development of a sensitive and specific qPCR assay in conjunction with propidium monoazide for enhanced detection of live Salmonella spp. in food

BACKGROUND: Although a variety of methodologies are available for detection of Salmonella, sensitive, specific, and efficient methods are urgently needed for differentiation of live Salmonella cells from dead cells in food and environmental samples. Propidium monoazide (PMA) can preferentially penet...

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Detalles Bibliográficos
Autores principales: Li, Baoguang, Chen, Jin-Qiang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4219372/
https://www.ncbi.nlm.nih.gov/pubmed/24289661
http://dx.doi.org/10.1186/1471-2180-13-273