Application of a SYBR® Green One Step Real-time RT-PCR Assay to Detect Type 1 Porcine Reproductive and Respiratory Syndrome Virus

The emergence in Japan of field isolates of type 1 porcine reproductive and respiratory syndrome virus (PRRSV) suggests problems with control. We therefore developed a one-step real-time reverse transcription polymerase chain reaction (qRT-PCR) with improved sensitivity that detects as little as 1 ×...

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Detalles Bibliográficos
Autores principales: ISEKI, Hiroshi, TAKAGI, Michihiro, KURODA, Yoshiko, KATSUDA, Ken, MIKAMI, Osamu, TSUNEMITSU, Hiroshi, YAMAKAWA, Makoto
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Japanese Society of Veterinary Science 2014
Materias:
Virology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4221178/
https://www.ncbi.nlm.nih.gov/pubmed/25047905
http://dx.doi.org/10.1292/jvms.14-0010

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4221178/
https://www.ncbi.nlm.nih.gov/pubmed/25047905
http://dx.doi.org/10.1292/jvms.14-0010

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