Cargando…
Transcriptional programs of lymphoid tissue capillary and high endothelium reveal control mechanisms for lymphocyte homing
Lymphocytes are recruited from blood by high-endothelial venules (HEVs). We performed transcriptomic analyses and identified molecular signatures that distinguish HEVs from capillary endothelium and that define tissue-specific HEV specialization. Capillaries displayed gene programs for vascular deve...
Autores principales: | , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
2014
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4222088/ https://www.ncbi.nlm.nih.gov/pubmed/25173345 http://dx.doi.org/10.1038/ni.2983 |
_version_ | 1782342977311473664 |
---|---|
author | Lee, Mike Kiefel, Helena LaJevic, Melissa D. Macauley, Matthew S. Kawashima, Hiroto O'Hara, Edward Pan, Junliang Paulson, James C. Butcher, Eugene C. |
author_facet | Lee, Mike Kiefel, Helena LaJevic, Melissa D. Macauley, Matthew S. Kawashima, Hiroto O'Hara, Edward Pan, Junliang Paulson, James C. Butcher, Eugene C. |
author_sort | Lee, Mike |
collection | PubMed |
description | Lymphocytes are recruited from blood by high-endothelial venules (HEVs). We performed transcriptomic analyses and identified molecular signatures that distinguish HEVs from capillary endothelium and that define tissue-specific HEV specialization. Capillaries displayed gene programs for vascular development. HEVs were enriched in genes for immune defense and lymphocyte migration. We identify capillary and HEV markers and candidate mechanisms for regulated lymphocyte recruitment including a lymph node HEV-selective transmembrane mucin; transcriptional control of functionally specialized carbohydrate ligands for lymphocyte L-selectin; HEV expression of molecules for transendothelial migration; and metabolic programs for lipid mediators of lymphocyte motility and chemotaxis. We also elucidate a carbohydrate recognition pathway that targets B cells to intestinal lymphoid tissues, defining CD22 as a lectin-homing receptor for mucosal HEVs. |
format | Online Article Text |
id | pubmed-4222088 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
record_format | MEDLINE/PubMed |
spelling | pubmed-42220882015-04-01 Transcriptional programs of lymphoid tissue capillary and high endothelium reveal control mechanisms for lymphocyte homing Lee, Mike Kiefel, Helena LaJevic, Melissa D. Macauley, Matthew S. Kawashima, Hiroto O'Hara, Edward Pan, Junliang Paulson, James C. Butcher, Eugene C. Nat Immunol Article Lymphocytes are recruited from blood by high-endothelial venules (HEVs). We performed transcriptomic analyses and identified molecular signatures that distinguish HEVs from capillary endothelium and that define tissue-specific HEV specialization. Capillaries displayed gene programs for vascular development. HEVs were enriched in genes for immune defense and lymphocyte migration. We identify capillary and HEV markers and candidate mechanisms for regulated lymphocyte recruitment including a lymph node HEV-selective transmembrane mucin; transcriptional control of functionally specialized carbohydrate ligands for lymphocyte L-selectin; HEV expression of molecules for transendothelial migration; and metabolic programs for lipid mediators of lymphocyte motility and chemotaxis. We also elucidate a carbohydrate recognition pathway that targets B cells to intestinal lymphoid tissues, defining CD22 as a lectin-homing receptor for mucosal HEVs. 2014-08-31 2014-10 /pmc/articles/PMC4222088/ /pubmed/25173345 http://dx.doi.org/10.1038/ni.2983 Text en http://www.nature.com/authors/editorial_policies/license.html#terms Users may view, print, copy, and download text and data-mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use:http://www.nature.com/authors/editorial_policies/license.html#terms |
spellingShingle | Article Lee, Mike Kiefel, Helena LaJevic, Melissa D. Macauley, Matthew S. Kawashima, Hiroto O'Hara, Edward Pan, Junliang Paulson, James C. Butcher, Eugene C. Transcriptional programs of lymphoid tissue capillary and high endothelium reveal control mechanisms for lymphocyte homing |
title | Transcriptional programs of lymphoid tissue capillary and high endothelium reveal control mechanisms for lymphocyte homing |
title_full | Transcriptional programs of lymphoid tissue capillary and high endothelium reveal control mechanisms for lymphocyte homing |
title_fullStr | Transcriptional programs of lymphoid tissue capillary and high endothelium reveal control mechanisms for lymphocyte homing |
title_full_unstemmed | Transcriptional programs of lymphoid tissue capillary and high endothelium reveal control mechanisms for lymphocyte homing |
title_short | Transcriptional programs of lymphoid tissue capillary and high endothelium reveal control mechanisms for lymphocyte homing |
title_sort | transcriptional programs of lymphoid tissue capillary and high endothelium reveal control mechanisms for lymphocyte homing |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4222088/ https://www.ncbi.nlm.nih.gov/pubmed/25173345 http://dx.doi.org/10.1038/ni.2983 |
work_keys_str_mv | AT leemike transcriptionalprogramsoflymphoidtissuecapillaryandhighendotheliumrevealcontrolmechanismsforlymphocytehoming AT kiefelhelena transcriptionalprogramsoflymphoidtissuecapillaryandhighendotheliumrevealcontrolmechanismsforlymphocytehoming AT lajevicmelissad transcriptionalprogramsoflymphoidtissuecapillaryandhighendotheliumrevealcontrolmechanismsforlymphocytehoming AT macauleymatthews transcriptionalprogramsoflymphoidtissuecapillaryandhighendotheliumrevealcontrolmechanismsforlymphocytehoming AT kawashimahiroto transcriptionalprogramsoflymphoidtissuecapillaryandhighendotheliumrevealcontrolmechanismsforlymphocytehoming AT oharaedward transcriptionalprogramsoflymphoidtissuecapillaryandhighendotheliumrevealcontrolmechanismsforlymphocytehoming AT panjunliang transcriptionalprogramsoflymphoidtissuecapillaryandhighendotheliumrevealcontrolmechanismsforlymphocytehoming AT paulsonjamesc transcriptionalprogramsoflymphoidtissuecapillaryandhighendotheliumrevealcontrolmechanismsforlymphocytehoming AT butchereugenec transcriptionalprogramsoflymphoidtissuecapillaryandhighendotheliumrevealcontrolmechanismsforlymphocytehoming |