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Validation of an ultrasensitive digital droplet PCR assay for HIV-2 plasma RNA quantification

INTRODUCTION: Low or undetectable plasma viral load (VL) using current qPCR assays is common for HIV-2 patients. Digital PCR is an emerging technology enabling more precision and reproducibility than qPCR at low DNA/RNA copy numbers. Available data related to digital droplet PCR (ddPCR, Bio-Rad) und...

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Detalles Bibliográficos
Autores principales: Ruelle, Jean, Yfantis, Vasilieios, Duquenne, Armelle, Goubau, Patrick
Formato: Online Artículo Texto
Lenguaje:English
Publicado: International AIDS Society 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4225411/
https://www.ncbi.nlm.nih.gov/pubmed/25397425
http://dx.doi.org/10.7448/IAS.17.4.19675