Experimental evaluation does not reveal a direct effect of microRNA from the callipyge locus on DLK1 expression

BACKGROUND: Polar overdominance at the ovine callipyge (CLPG) locus involves the post-transcriptional trans-inhibition of DLK1 in skeletal muscle of CLPG/CLPG sheep. The abundant maternally expressed microRNAs (miRNAs) mapping to the imprinted DLK1-GTL2 domain are prime candidate mediators of this t...

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Autores principales: Cheng, Huijun, Xu, Xuewen, Hadfield, Tracy, Cockett, Noelle, Charlier, Carole, Georges, Michel, Takeda, Haruko
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2014
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4226911/
https://www.ncbi.nlm.nih.gov/pubmed/25359221
http://dx.doi.org/10.1186/1471-2164-15-944
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author Cheng, Huijun
Xu, Xuewen
Hadfield, Tracy
Cockett, Noelle
Charlier, Carole
Georges, Michel
Takeda, Haruko
author_facet Cheng, Huijun
Xu, Xuewen
Hadfield, Tracy
Cockett, Noelle
Charlier, Carole
Georges, Michel
Takeda, Haruko
author_sort Cheng, Huijun
collection PubMed
description BACKGROUND: Polar overdominance at the ovine callipyge (CLPG) locus involves the post-transcriptional trans-inhibition of DLK1 in skeletal muscle of CLPG/CLPG sheep. The abundant maternally expressed microRNAs (miRNAs) mapping to the imprinted DLK1-GTL2 domain are prime candidate mediators of this trans-effect. RESULTS: We have tested the affinity of 121 miRNAs processed from this locus for DLK1 by co-transfecting COS1 cells with a vector expressing the full-length ovine DLK1 with corresponding mimic miRNAs. None of the tested miRNAs was able to down regulate DLK1 to the extent observed in vivo. CONCLUSIONS: This suggests that other factors, with or without these miRNAs, are involved in mediating the observed trans-effect. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/1471-2164-15-944) contains supplementary material, which is available to authorized users.
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spelling pubmed-42269112014-11-12 Experimental evaluation does not reveal a direct effect of microRNA from the callipyge locus on DLK1 expression Cheng, Huijun Xu, Xuewen Hadfield, Tracy Cockett, Noelle Charlier, Carole Georges, Michel Takeda, Haruko BMC Genomics Research Article BACKGROUND: Polar overdominance at the ovine callipyge (CLPG) locus involves the post-transcriptional trans-inhibition of DLK1 in skeletal muscle of CLPG/CLPG sheep. The abundant maternally expressed microRNAs (miRNAs) mapping to the imprinted DLK1-GTL2 domain are prime candidate mediators of this trans-effect. RESULTS: We have tested the affinity of 121 miRNAs processed from this locus for DLK1 by co-transfecting COS1 cells with a vector expressing the full-length ovine DLK1 with corresponding mimic miRNAs. None of the tested miRNAs was able to down regulate DLK1 to the extent observed in vivo. CONCLUSIONS: This suggests that other factors, with or without these miRNAs, are involved in mediating the observed trans-effect. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/1471-2164-15-944) contains supplementary material, which is available to authorized users. BioMed Central 2014-10-30 /pmc/articles/PMC4226911/ /pubmed/25359221 http://dx.doi.org/10.1186/1471-2164-15-944 Text en © Cheng et al.; licensee BioMed Central Ltd. 2014 This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Cheng, Huijun
Xu, Xuewen
Hadfield, Tracy
Cockett, Noelle
Charlier, Carole
Georges, Michel
Takeda, Haruko
Experimental evaluation does not reveal a direct effect of microRNA from the callipyge locus on DLK1 expression
title Experimental evaluation does not reveal a direct effect of microRNA from the callipyge locus on DLK1 expression
title_full Experimental evaluation does not reveal a direct effect of microRNA from the callipyge locus on DLK1 expression
title_fullStr Experimental evaluation does not reveal a direct effect of microRNA from the callipyge locus on DLK1 expression
title_full_unstemmed Experimental evaluation does not reveal a direct effect of microRNA from the callipyge locus on DLK1 expression
title_short Experimental evaluation does not reveal a direct effect of microRNA from the callipyge locus on DLK1 expression
title_sort experimental evaluation does not reveal a direct effect of microrna from the callipyge locus on dlk1 expression
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4226911/
https://www.ncbi.nlm.nih.gov/pubmed/25359221
http://dx.doi.org/10.1186/1471-2164-15-944
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