PaperClip: rapid multi-part DNA assembly from existing libraries

Assembly of DNA ‘parts’ to create larger constructs is an essential enabling technique for bioengineering and synthetic biology. Here we describe a simple method, PaperClip, which allows flexible assembly of multiple DNA parts from currently existing libraries cloned in any vector. No restriction en...

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Detalles Bibliográficos
Autores principales: Trubitsyna, Maryia, Michlewski, Gracjan, Cai, Yizhi, Elfick, Alistair, French, Christopher E.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2014
Materias:
Methods Online
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4227759/
https://www.ncbi.nlm.nih.gov/pubmed/25200084
http://dx.doi.org/10.1093/nar/gku829

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4227759/
https://www.ncbi.nlm.nih.gov/pubmed/25200084
http://dx.doi.org/10.1093/nar/gku829

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