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PaperClip: rapid multi-part DNA assembly from existing libraries
Assembly of DNA ‘parts’ to create larger constructs is an essential enabling technique for bioengineering and synthetic biology. Here we describe a simple method, PaperClip, which allows flexible assembly of multiple DNA parts from currently existing libraries cloned in any vector. No restriction en...
Autores principales: | Trubitsyna, Maryia, Michlewski, Gracjan, Cai, Yizhi, Elfick, Alistair, French, Christopher E. |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4227759/ https://www.ncbi.nlm.nih.gov/pubmed/25200084 http://dx.doi.org/10.1093/nar/gku829 |
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