Determination of Glutamate Dehydrogenase Activity and Its Kinetics in Mouse Tissues using Metabolic Mapping (Quantitative Enzyme Histochemistry)

Glutamate dehydrogenase (GDH) catalyses the reversible conversion of glutamate into α-ketoglutarate with the concomitant reduction of NAD(P)(+) to NAD(P)H or vice versa. GDH activity is subject to complex allosteric regulation including substrate inhibition. To determine GDH kinetics in situ, we ass...

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Detalles Bibliográficos
Autores principales: Botman, Dennis, Tigchelaar, Wikky, Van Noorden, Cornelis J.F.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: SAGE Publications 2014
Materias:
Articles
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4230541/
https://www.ncbi.nlm.nih.gov/pubmed/25124006
http://dx.doi.org/10.1369/0022155414549071

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4230541/
https://www.ncbi.nlm.nih.gov/pubmed/25124006
http://dx.doi.org/10.1369/0022155414549071

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