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A novel pathway of rapid TLR-triggered activation of integrin-dependent leukocyte adhesion that requires Rap1 GTPase

Rapid β2-integrin activation is indispensable for leukocyte adhesion and recruitment to sites of infection and is mediated by chemokine- or P-selectin glycoprotein ligand-1–induced inside-out signaling. Here we uncovered a novel pathway for rapid activation of integrin-dependent leukocyte adhesion,...

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Autores principales: Chung, Kyoung-Jin, Mitroulis, Ioannis, Wiessner, Johannes R., Zheng, Ying Yi, Siegert, Gabriele, Sperandio, Markus, Chavakis, Triantafyllos
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The American Society for Cell Biology 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4230584/
https://www.ncbi.nlm.nih.gov/pubmed/25057020
http://dx.doi.org/10.1091/mbc.E14-04-0867
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author Chung, Kyoung-Jin
Mitroulis, Ioannis
Wiessner, Johannes R.
Zheng, Ying Yi
Siegert, Gabriele
Sperandio, Markus
Chavakis, Triantafyllos
author_facet Chung, Kyoung-Jin
Mitroulis, Ioannis
Wiessner, Johannes R.
Zheng, Ying Yi
Siegert, Gabriele
Sperandio, Markus
Chavakis, Triantafyllos
author_sort Chung, Kyoung-Jin
collection PubMed
description Rapid β2-integrin activation is indispensable for leukocyte adhesion and recruitment to sites of infection and is mediated by chemokine- or P-selectin glycoprotein ligand-1–induced inside-out signaling. Here we uncovered a novel pathway for rapid activation of integrin-dependent leukocyte adhesion, triggered by toll-like receptor (TLR)–mediated signaling. TLR2 or TLR5 ligation rapidly activated integrin-dependent leukocyte adhesion to immobilized ICAM-1 and fibronectin. Consistently, in vivo administration of the TLR2-ligand Pam3CSK4 increased integrin-dependent slow rolling and adhesion to endothelium within minutes, as identified by intravital microscopy in the cremaster model. TLR2 and TLR5 ligation increased β2-integrin affinity, as assessed by the detection of activation-dependent neoepitopes. TLR2- and TLR5-triggered integrin activation in leukocytes required enhanced Rap1 GTPase activity, which was mediated by Rac1 activation and NADPH oxidase-2–dependent reactive oxygen species production. This novel direct pathway linking initial pathogen recognition by TLRs to rapid β2-integrin activation may critically regulate acute leukocyte infiltration to sites of pathogen invasion.
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spelling pubmed-42305842014-12-16 A novel pathway of rapid TLR-triggered activation of integrin-dependent leukocyte adhesion that requires Rap1 GTPase Chung, Kyoung-Jin Mitroulis, Ioannis Wiessner, Johannes R. Zheng, Ying Yi Siegert, Gabriele Sperandio, Markus Chavakis, Triantafyllos Mol Biol Cell Articles Rapid β2-integrin activation is indispensable for leukocyte adhesion and recruitment to sites of infection and is mediated by chemokine- or P-selectin glycoprotein ligand-1–induced inside-out signaling. Here we uncovered a novel pathway for rapid activation of integrin-dependent leukocyte adhesion, triggered by toll-like receptor (TLR)–mediated signaling. TLR2 or TLR5 ligation rapidly activated integrin-dependent leukocyte adhesion to immobilized ICAM-1 and fibronectin. Consistently, in vivo administration of the TLR2-ligand Pam3CSK4 increased integrin-dependent slow rolling and adhesion to endothelium within minutes, as identified by intravital microscopy in the cremaster model. TLR2 and TLR5 ligation increased β2-integrin affinity, as assessed by the detection of activation-dependent neoepitopes. TLR2- and TLR5-triggered integrin activation in leukocytes required enhanced Rap1 GTPase activity, which was mediated by Rac1 activation and NADPH oxidase-2–dependent reactive oxygen species production. This novel direct pathway linking initial pathogen recognition by TLRs to rapid β2-integrin activation may critically regulate acute leukocyte infiltration to sites of pathogen invasion. The American Society for Cell Biology 2014-10-01 /pmc/articles/PMC4230584/ /pubmed/25057020 http://dx.doi.org/10.1091/mbc.E14-04-0867 Text en © 2014 Chung et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0). “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society of Cell Biology.
spellingShingle Articles
Chung, Kyoung-Jin
Mitroulis, Ioannis
Wiessner, Johannes R.
Zheng, Ying Yi
Siegert, Gabriele
Sperandio, Markus
Chavakis, Triantafyllos
A novel pathway of rapid TLR-triggered activation of integrin-dependent leukocyte adhesion that requires Rap1 GTPase
title A novel pathway of rapid TLR-triggered activation of integrin-dependent leukocyte adhesion that requires Rap1 GTPase
title_full A novel pathway of rapid TLR-triggered activation of integrin-dependent leukocyte adhesion that requires Rap1 GTPase
title_fullStr A novel pathway of rapid TLR-triggered activation of integrin-dependent leukocyte adhesion that requires Rap1 GTPase
title_full_unstemmed A novel pathway of rapid TLR-triggered activation of integrin-dependent leukocyte adhesion that requires Rap1 GTPase
title_short A novel pathway of rapid TLR-triggered activation of integrin-dependent leukocyte adhesion that requires Rap1 GTPase
title_sort novel pathway of rapid tlr-triggered activation of integrin-dependent leukocyte adhesion that requires rap1 gtpase
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4230584/
https://www.ncbi.nlm.nih.gov/pubmed/25057020
http://dx.doi.org/10.1091/mbc.E14-04-0867
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