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Molecular counting by photobleaching in protein complexes with many subunits: best practices and application to the cellulose synthesis complex

The constituents of large, multisubunit protein complexes dictate their functions in cells, but determining their precise molecular makeup in vivo is challenging. One example of such a complex is the cellulose synthesis complex (CSC), which in plants synthesizes cellulose, the most abundant biopolym...

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Detalles Bibliográficos
Autores principales: Chen, Yalei, Deffenbaugh, Nathan C., Anderson, Charles T., Hancock, William O.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The American Society for Cell Biology 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4230622/
https://www.ncbi.nlm.nih.gov/pubmed/25232006
http://dx.doi.org/10.1091/mbc.E14-06-1146