NADH-dependent biosensor in Saccharomyces cerevisiae: principle and validation at the single cell level

A reporter system was constructed to measure perturbations in the NADH/NAD(+) co-factor balance in yeast, by using the green fluorescent protein gene under the control of the GPD2 promoter that is induced under conditions of excess of NADH. High fluorescence levels were obtained in a glycerol 3-phos...

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Detalles Bibliográficos
Autores principales: Knudsen, Jan Dines, Carlquist, Magnus, Gorwa-Grauslund, Marie
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer 2014
Materias:
Original Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4230897/
https://www.ncbi.nlm.nih.gov/pubmed/25401080
http://dx.doi.org/10.1186/s13568-014-0081-4

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4230897/
https://www.ncbi.nlm.nih.gov/pubmed/25401080
http://dx.doi.org/10.1186/s13568-014-0081-4

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