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NADH-dependent biosensor in Saccharomyces cerevisiae: principle and validation at the single cell level
A reporter system was constructed to measure perturbations in the NADH/NAD(+) co-factor balance in yeast, by using the green fluorescent protein gene under the control of the GPD2 promoter that is induced under conditions of excess of NADH. High fluorescence levels were obtained in a glycerol 3-phos...
| Autores principales: | , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Springer
2014
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4230897/ https://www.ncbi.nlm.nih.gov/pubmed/25401080 http://dx.doi.org/10.1186/s13568-014-0081-4 |