Dual gene expression cassette vectors with antibiotic selection markers for engineering in Saccharomyces cerevisiae

BACKGROUND: Manipulations in Saccharomyces cerevisiae classically depend on use of auxotrophy selection markers. There are several disadvantages to this in a microbial cell factory setting: (1) auxotrophies must first be engineered in prototrophic strains, and many industrial strains are polyploid/a...

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Detalles Bibliográficos
Autores principales: Vickers, Claudia E, Bydder, Sarah F, Zhou, Yuchan, Nielsen, Lars K
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2013
Materias:
Technical Notes
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4231455/
https://www.ncbi.nlm.nih.gov/pubmed/24161108
http://dx.doi.org/10.1186/1475-2859-12-96

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4231455/
https://www.ncbi.nlm.nih.gov/pubmed/24161108
http://dx.doi.org/10.1186/1475-2859-12-96

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