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Dual gene expression cassette vectors with antibiotic selection markers for engineering in Saccharomyces cerevisiae

BACKGROUND: Manipulations in Saccharomyces cerevisiae classically depend on use of auxotrophy selection markers. There are several disadvantages to this in a microbial cell factory setting: (1) auxotrophies must first be engineered in prototrophic strains, and many industrial strains are polyploid/a...

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Detalles Bibliográficos
Autores principales:	Vickers, Claudia E, Bydder, Sarah F, Zhou, Yuchan, Nielsen, Lars K
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	BioMed Central 2013
Materias:	Technical Notes
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4231455/ https://www.ncbi.nlm.nih.gov/pubmed/24161108 http://dx.doi.org/10.1186/1475-2859-12-96

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