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Multiplex CRISPR/Cas9-based genome engineering from a single lentiviral vector

Engineered DNA-binding proteins that manipulate the human genome and transcriptome have enabled rapid advances in biomedical research. In particular, the RNA-guided CRISPR/Cas9 system has recently been engineered to create site-specific double-strand breaks for genome editing or to direct targeted t...

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Detalles Bibliográficos
Autores principales:	Kabadi, Ami M., Ousterout, David G., Hilton, Isaac B., Gersbach, Charles A.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Oxford University Press 2014
Materias:	Methods Online
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4231726/ https://www.ncbi.nlm.nih.gov/pubmed/25122746 http://dx.doi.org/10.1093/nar/gku749

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