Weak base pairing in both seed and 3′ regions reduces RNAi off-targets and enhances si/shRNA designs

The use of RNA interference is becoming routine in scientific discovery and treatment of human disease. However, its applications are hampered by unwanted effects, particularly off-targeting through miRNA-like pathways. Recent studies suggest that the efficacy of such off-targeting might be dependen...

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Detalles Bibliográficos
Autores principales: Gu, Shuo, Zhang, Yue, Jin, Lan, Huang, Yong, Zhang, Feijie, Bassik, Michael C., Kampmann, Martin, Kay, Mark A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2014
Materias:
RNA
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4231738/
https://www.ncbi.nlm.nih.gov/pubmed/25270879
http://dx.doi.org/10.1093/nar/gku854

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4231738/
https://www.ncbi.nlm.nih.gov/pubmed/25270879
http://dx.doi.org/10.1093/nar/gku854

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