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Weak base pairing in both seed and 3′ regions reduces RNAi off-targets and enhances si/shRNA designs
The use of RNA interference is becoming routine in scientific discovery and treatment of human disease. However, its applications are hampered by unwanted effects, particularly off-targeting through miRNA-like pathways. Recent studies suggest that the efficacy of such off-targeting might be dependen...
| Autores principales: | , , , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Oxford University Press
2014
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4231738/ https://www.ncbi.nlm.nih.gov/pubmed/25270879 http://dx.doi.org/10.1093/nar/gku854 |