A Method for Sporulating Budding Yeast Cells That Allows for Unbiased Identification of Kinase Substrates Using Stable Isotope Labeling by Amino Acids in Cell Culture

Quantitative proteomics has been widely used to elucidate many cellular processes. In particular, stable isotope labeling by amino acids in cell culture (SILAC) has been instrumental in improving the quality of data generated from quantitative high-throughput proteomic studies. SILAC uses the cell’s...

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Detalles Bibliográficos
Autores principales: Suhandynata, Ray, Liang, Jason, Albuquerque, Claudio. P., Zhou, Huilin, Hollingsworth, Nancy M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Genetics Society of America 2014
Materias:
Investigations
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4232538/
https://www.ncbi.nlm.nih.gov/pubmed/25168012
http://dx.doi.org/10.1534/g3.114.013888

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4232538/
https://www.ncbi.nlm.nih.gov/pubmed/25168012
http://dx.doi.org/10.1534/g3.114.013888

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