Structures of Naturally Evolved CUP1 Tandem Arrays in Yeast Indicate That These Arrays Are Generated by Unequal Nonhomologous Recombination

An important issue in genome evolution is the mechanism by which tandem duplications are generated from single-copy genes. In the yeast Saccharomyces cerevisiae, most strains contain tandemly duplicated copies of CUP1, a gene that encodes a copper-binding metallothionein. By screening 101 natural is...

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Autores principales: Zhao, Ying, Strope, Pooja K., Kozmin, Stanislav G., McCusker, John H., Dietrich, Fred S., Kokoska, Robert J., Petes, Thomas D.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Genetics Society of America 2014
Materias:
Investigations
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4232551/
https://www.ncbi.nlm.nih.gov/pubmed/25236733
http://dx.doi.org/10.1534/g3.114.012922
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author Zhao, Ying
Strope, Pooja K.
Kozmin, Stanislav G.
McCusker, John H.
Dietrich, Fred S.
Kokoska, Robert J.
Petes, Thomas D.
author_facet Zhao, Ying
Strope, Pooja K.
Kozmin, Stanislav G.
McCusker, John H.
Dietrich, Fred S.
Kokoska, Robert J.
Petes, Thomas D.
author_sort Zhao, Ying
collection PubMed
description An important issue in genome evolution is the mechanism by which tandem duplications are generated from single-copy genes. In the yeast Saccharomyces cerevisiae, most strains contain tandemly duplicated copies of CUP1, a gene that encodes a copper-binding metallothionein. By screening 101 natural isolates of S. cerevisiae, we identified five different types of CUP1-containing repeats, as well as strains that only had one copy of CUP1. A comparison of the DNA sequences of these strains indicates that the CUP1 tandem arrays were generated by unequal nonhomologous recombination events from strains that had one CUP1 gene.
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spelling pubmed-42325512014-11-18 Structures of Naturally Evolved CUP1 Tandem Arrays in Yeast Indicate That These Arrays Are Generated by Unequal Nonhomologous Recombination Zhao, Ying Strope, Pooja K. Kozmin, Stanislav G. McCusker, John H. Dietrich, Fred S. Kokoska, Robert J. Petes, Thomas D. G3 (Bethesda) Investigations An important issue in genome evolution is the mechanism by which tandem duplications are generated from single-copy genes. In the yeast Saccharomyces cerevisiae, most strains contain tandemly duplicated copies of CUP1, a gene that encodes a copper-binding metallothionein. By screening 101 natural isolates of S. cerevisiae, we identified five different types of CUP1-containing repeats, as well as strains that only had one copy of CUP1. A comparison of the DNA sequences of these strains indicates that the CUP1 tandem arrays were generated by unequal nonhomologous recombination events from strains that had one CUP1 gene. Genetics Society of America 2014-09-17 /pmc/articles/PMC4232551/ /pubmed/25236733 http://dx.doi.org/10.1534/g3.114.012922 Text en Copyright © 2014 Zhao et al. http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution Unported License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Investigations
Zhao, Ying
Strope, Pooja K.
Kozmin, Stanislav G.
McCusker, John H.
Dietrich, Fred S.
Kokoska, Robert J.
Petes, Thomas D.
Structures of Naturally Evolved CUP1 Tandem Arrays in Yeast Indicate That These Arrays Are Generated by Unequal Nonhomologous Recombination
title Structures of Naturally Evolved CUP1 Tandem Arrays in Yeast Indicate That These Arrays Are Generated by Unequal Nonhomologous Recombination
title_full Structures of Naturally Evolved CUP1 Tandem Arrays in Yeast Indicate That These Arrays Are Generated by Unequal Nonhomologous Recombination
title_fullStr Structures of Naturally Evolved CUP1 Tandem Arrays in Yeast Indicate That These Arrays Are Generated by Unequal Nonhomologous Recombination
title_full_unstemmed Structures of Naturally Evolved CUP1 Tandem Arrays in Yeast Indicate That These Arrays Are Generated by Unequal Nonhomologous Recombination
title_short Structures of Naturally Evolved CUP1 Tandem Arrays in Yeast Indicate That These Arrays Are Generated by Unequal Nonhomologous Recombination
title_sort structures of naturally evolved cup1 tandem arrays in yeast indicate that these arrays are generated by unequal nonhomologous recombination
topic Investigations
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4232551/
https://www.ncbi.nlm.nih.gov/pubmed/25236733
http://dx.doi.org/10.1534/g3.114.012922
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