Self-assembled monolayers improve protein distribution on holey carbon cryo-EM supports

Poor partitioning of macromolecules into the holes of holey carbon support grids frequently limits structural determination by single particle cryo-electron microscopy (cryo-EM). Here, we present a method to deposit, on gold-coated carbon grids, a self-assembled monolayer whose surface properties ca...

Descripción completa

Detalles Bibliográficos
Autores principales: Meyerson, Joel R., Rao, Prashant, Kumar, Janesh, Chittori, Sagar, Banerjee, Soojay, Pierson, Jason, Mayer, Mark L., Subramaniam, Sriram
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2014
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4235105/
https://www.ncbi.nlm.nih.gov/pubmed/25403871
http://dx.doi.org/10.1038/srep07084

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4235105/
https://www.ncbi.nlm.nih.gov/pubmed/25403871
http://dx.doi.org/10.1038/srep07084

Ejemplares similares