Self-assembled monolayers improve protein distribution on holey carbon cryo-EM supports

Poor partitioning of macromolecules into the holes of holey carbon support grids frequently limits structural determination by single particle cryo-electron microscopy (cryo-EM). Here, we present a method to deposit, on gold-coated carbon grids, a self-assembled monolayer whose surface properties ca...

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Autores principales: Meyerson, Joel R., Rao, Prashant, Kumar, Janesh, Chittori, Sagar, Banerjee, Soojay, Pierson, Jason, Mayer, Mark L., Subramaniam, Sriram
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2014
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4235105/
https://www.ncbi.nlm.nih.gov/pubmed/25403871
http://dx.doi.org/10.1038/srep07084
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author Meyerson, Joel R.
Rao, Prashant
Kumar, Janesh
Chittori, Sagar
Banerjee, Soojay
Pierson, Jason
Mayer, Mark L.
Subramaniam, Sriram
author_facet Meyerson, Joel R.
Rao, Prashant
Kumar, Janesh
Chittori, Sagar
Banerjee, Soojay
Pierson, Jason
Mayer, Mark L.
Subramaniam, Sriram
author_sort Meyerson, Joel R.
collection PubMed
description Poor partitioning of macromolecules into the holes of holey carbon support grids frequently limits structural determination by single particle cryo-electron microscopy (cryo-EM). Here, we present a method to deposit, on gold-coated carbon grids, a self-assembled monolayer whose surface properties can be controlled by chemical modification. We demonstrate the utility of this approach to drive partitioning of ionotropic glutamate receptors into the holes, thereby enabling 3D structural analysis using cryo-EM methods.
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spelling pubmed-42351052014-11-25 Self-assembled monolayers improve protein distribution on holey carbon cryo-EM supports Meyerson, Joel R. Rao, Prashant Kumar, Janesh Chittori, Sagar Banerjee, Soojay Pierson, Jason Mayer, Mark L. Subramaniam, Sriram Sci Rep Article Poor partitioning of macromolecules into the holes of holey carbon support grids frequently limits structural determination by single particle cryo-electron microscopy (cryo-EM). Here, we present a method to deposit, on gold-coated carbon grids, a self-assembled monolayer whose surface properties can be controlled by chemical modification. We demonstrate the utility of this approach to drive partitioning of ionotropic glutamate receptors into the holes, thereby enabling 3D structural analysis using cryo-EM methods. Nature Publishing Group 2014-11-18 /pmc/articles/PMC4235105/ /pubmed/25403871 http://dx.doi.org/10.1038/srep07084 Text en Copyright © 2014, Macmillan Publishers Limited. All rights reserved http://creativecommons.org/licenses/by-nc-nd/4.0/ This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder in order to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/4.0/
spellingShingle Article
Meyerson, Joel R.
Rao, Prashant
Kumar, Janesh
Chittori, Sagar
Banerjee, Soojay
Pierson, Jason
Mayer, Mark L.
Subramaniam, Sriram
Self-assembled monolayers improve protein distribution on holey carbon cryo-EM supports
title Self-assembled monolayers improve protein distribution on holey carbon cryo-EM supports
title_full Self-assembled monolayers improve protein distribution on holey carbon cryo-EM supports
title_fullStr Self-assembled monolayers improve protein distribution on holey carbon cryo-EM supports
title_full_unstemmed Self-assembled monolayers improve protein distribution on holey carbon cryo-EM supports
title_short Self-assembled monolayers improve protein distribution on holey carbon cryo-EM supports
title_sort self-assembled monolayers improve protein distribution on holey carbon cryo-em supports
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4235105/
https://www.ncbi.nlm.nih.gov/pubmed/25403871
http://dx.doi.org/10.1038/srep07084
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