Oligoribonucleotide (ORN) Interference-PCR (ORNi-PCR): A Simple Method for Suppressing PCR Amplification of Specific DNA Sequences Using ORNs

Polymerase chain reaction (PCR) amplification of multiple templates using common primers is used in a wide variety of molecular biological techniques. However, abundant templates sometimes obscure the amplification of minor species containing the same primer sequences. To overcome this challenge, we...

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Detalles Bibliográficos
Autores principales: Tanigawa, Naoki, Fujita, Toshitsugu, Fujii, Hodaka
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2014
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4236179/
https://www.ncbi.nlm.nih.gov/pubmed/25405983
http://dx.doi.org/10.1371/journal.pone.0113345

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4236179/
https://www.ncbi.nlm.nih.gov/pubmed/25405983
http://dx.doi.org/10.1371/journal.pone.0113345

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