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Duration of the unconditioned stimulus in appetitive conditioning of honeybees differentially impacts learning, long-term memory strength, and the underlying protein synthesis

This study examines the role of stimulus duration in learning and memory formation of honeybees (Apis mellifera). In classical appetitive conditioning honeybees learn the association between an initially neutral, conditioned stimulus (CS) and the occurrence of a meaningful stimulus, the unconditione...

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Autores principales: Marter, Kathrin, Grauel, M. Katharina, Lewa, Carmen, Morgenstern, Laura, Buckemüller, Christina, Heufelder, Karin, Ganz, Marion, Eisenhardt, Dorothea
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cold Spring Harbor Laboratory Press 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4236413/
https://www.ncbi.nlm.nih.gov/pubmed/25403456
http://dx.doi.org/10.1101/lm.035600.114
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author Marter, Kathrin
Grauel, M. Katharina
Lewa, Carmen
Morgenstern, Laura
Buckemüller, Christina
Heufelder, Karin
Ganz, Marion
Eisenhardt, Dorothea
author_facet Marter, Kathrin
Grauel, M. Katharina
Lewa, Carmen
Morgenstern, Laura
Buckemüller, Christina
Heufelder, Karin
Ganz, Marion
Eisenhardt, Dorothea
author_sort Marter, Kathrin
collection PubMed
description This study examines the role of stimulus duration in learning and memory formation of honeybees (Apis mellifera). In classical appetitive conditioning honeybees learn the association between an initially neutral, conditioned stimulus (CS) and the occurrence of a meaningful stimulus, the unconditioned stimulus (US). Thereby the CS becomes a predictor for the US eliciting a conditioned response (CR). Here we study the role of US duration in classical conditioning by examining honeybees conditioned with different US durations. We quantify the CR during acquisition, memory retention, and extinction of the early long-term memory (eLTM), and examine the molecular mechanisms of eLTM by interfering with protein synthesis. We find that the US duration affects neither the probability nor the strength of the CR during acquisition, eLTM retention, and extinction 24 h after conditioning. However, we find that the resistance to extinction 24 h after conditioning is susceptible to protein synthesis inhibition depending on the US duration. We conclude that the US duration does not affect the predictability of the US but modulates the protein synthesis underlying the eLTM's strength. Thus, the US duration differentially impacts learning, eLTM strength, and its underlying protein synthesis.
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spelling pubmed-42364132015-12-01 Duration of the unconditioned stimulus in appetitive conditioning of honeybees differentially impacts learning, long-term memory strength, and the underlying protein synthesis Marter, Kathrin Grauel, M. Katharina Lewa, Carmen Morgenstern, Laura Buckemüller, Christina Heufelder, Karin Ganz, Marion Eisenhardt, Dorothea Learn Mem Research This study examines the role of stimulus duration in learning and memory formation of honeybees (Apis mellifera). In classical appetitive conditioning honeybees learn the association between an initially neutral, conditioned stimulus (CS) and the occurrence of a meaningful stimulus, the unconditioned stimulus (US). Thereby the CS becomes a predictor for the US eliciting a conditioned response (CR). Here we study the role of US duration in classical conditioning by examining honeybees conditioned with different US durations. We quantify the CR during acquisition, memory retention, and extinction of the early long-term memory (eLTM), and examine the molecular mechanisms of eLTM by interfering with protein synthesis. We find that the US duration affects neither the probability nor the strength of the CR during acquisition, eLTM retention, and extinction 24 h after conditioning. However, we find that the resistance to extinction 24 h after conditioning is susceptible to protein synthesis inhibition depending on the US duration. We conclude that the US duration does not affect the predictability of the US but modulates the protein synthesis underlying the eLTM's strength. Thus, the US duration differentially impacts learning, eLTM strength, and its underlying protein synthesis. Cold Spring Harbor Laboratory Press 2014-12 /pmc/articles/PMC4236413/ /pubmed/25403456 http://dx.doi.org/10.1101/lm.035600.114 Text en © 2014 Marter et al.; Published by Cold Spring Harbor Laboratory Press http://creativecommons.org/licenses/by-nc/4.0/ This article is distributed exclusively by Cold Spring Harbor Laboratory Press for the first 12 months after the full-issue publication date (see http://learnmem.cshlp.org/site/misc/terms.xhtml). After 12 months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.
spellingShingle Research
Marter, Kathrin
Grauel, M. Katharina
Lewa, Carmen
Morgenstern, Laura
Buckemüller, Christina
Heufelder, Karin
Ganz, Marion
Eisenhardt, Dorothea
Duration of the unconditioned stimulus in appetitive conditioning of honeybees differentially impacts learning, long-term memory strength, and the underlying protein synthesis
title Duration of the unconditioned stimulus in appetitive conditioning of honeybees differentially impacts learning, long-term memory strength, and the underlying protein synthesis
title_full Duration of the unconditioned stimulus in appetitive conditioning of honeybees differentially impacts learning, long-term memory strength, and the underlying protein synthesis
title_fullStr Duration of the unconditioned stimulus in appetitive conditioning of honeybees differentially impacts learning, long-term memory strength, and the underlying protein synthesis
title_full_unstemmed Duration of the unconditioned stimulus in appetitive conditioning of honeybees differentially impacts learning, long-term memory strength, and the underlying protein synthesis
title_short Duration of the unconditioned stimulus in appetitive conditioning of honeybees differentially impacts learning, long-term memory strength, and the underlying protein synthesis
title_sort duration of the unconditioned stimulus in appetitive conditioning of honeybees differentially impacts learning, long-term memory strength, and the underlying protein synthesis
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4236413/
https://www.ncbi.nlm.nih.gov/pubmed/25403456
http://dx.doi.org/10.1101/lm.035600.114
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