A versatile and highly efficient method for scarless genome editing in Escherichia coli and Salmonella enterica

BACKGROUND: Recently developed methods for genome editing in bacteria take advantage of the introduction of double-strand breaks by I-SceI in a mutation cassette to select for cells in which homologous recombination has healed the break and introduced a desired mutation. This elegantly designed meth...

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Detalles Bibliográficos
Autores principales: Kim, Juhan, Webb, Anthony M, Kershner, Jamie P, Blaskowski, Stephen, Copley, Shelley D
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2014
Materias:
Methodology Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4236582/
https://www.ncbi.nlm.nih.gov/pubmed/25255806
http://dx.doi.org/10.1186/1472-6750-14-84

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4236582/
https://www.ncbi.nlm.nih.gov/pubmed/25255806
http://dx.doi.org/10.1186/1472-6750-14-84

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