An eIF4E-interacting peptide induces cell death in cancer cell lines

The eukaryotic initiation factor eIF4E is essential for cap-dependent initiation of translation in eukaryotes. Abnormal regulation of eIF4E has been implicated in oncogenic transformation. We developed an eIF4E-binding peptide derived from Angel1, a partner of eIF4E that we recently identified. We s...

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Autores principales: Masse, M, Glippa, V, Saad, H, Le Bloas, R, Gauffeny, I, Berthou, C, Czjzek, M, Cormier, P, Cosson, B
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2014
Materias:
Original Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4237268/
https://www.ncbi.nlm.nih.gov/pubmed/25356869
http://dx.doi.org/10.1038/cddis.2014.457
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author Masse, M
Glippa, V
Saad, H
Le Bloas, R
Gauffeny, I
Berthou, C
Czjzek, M
Cormier, P
Cosson, B
author_facet Masse, M
Glippa, V
Saad, H
Le Bloas, R
Gauffeny, I
Berthou, C
Czjzek, M
Cormier, P
Cosson, B
author_sort Masse, M
collection PubMed
description The eukaryotic initiation factor eIF4E is essential for cap-dependent initiation of translation in eukaryotes. Abnormal regulation of eIF4E has been implicated in oncogenic transformation. We developed an eIF4E-binding peptide derived from Angel1, a partner of eIF4E that we recently identified. We show here that this peptide fused to a penetratin motif causes drastic and rapid cell death in several epithelial cancer cell lines. This necrotic cell death was characterized by a drop in ATP levels with F-actin network injury being a key step in extensive plasma membrane blebbing and membrane permeabilization. This synthetic eIF4E-binding peptide provides a candidate pharmacophore for a promising new cancer therapy strategy.
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spelling pubmed-42372682014-11-26 An eIF4E-interacting peptide induces cell death in cancer cell lines Masse, M Glippa, V Saad, H Le Bloas, R Gauffeny, I Berthou, C Czjzek, M Cormier, P Cosson, B Cell Death Dis Original Article The eukaryotic initiation factor eIF4E is essential for cap-dependent initiation of translation in eukaryotes. Abnormal regulation of eIF4E has been implicated in oncogenic transformation. We developed an eIF4E-binding peptide derived from Angel1, a partner of eIF4E that we recently identified. We show here that this peptide fused to a penetratin motif causes drastic and rapid cell death in several epithelial cancer cell lines. This necrotic cell death was characterized by a drop in ATP levels with F-actin network injury being a key step in extensive plasma membrane blebbing and membrane permeabilization. This synthetic eIF4E-binding peptide provides a candidate pharmacophore for a promising new cancer therapy strategy. Nature Publishing Group 2014-10 2014-10-30 /pmc/articles/PMC4237268/ /pubmed/25356869 http://dx.doi.org/10.1038/cddis.2014.457 Text en Copyright © 2014 Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ Cell Death and Disease is an open-access journal published by Nature Publishing Group. This work is licensed under a Creative Commons Attribution 4.0 International Licence. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons licence, users will need to obtain permission from the licence holder to reproduce the material. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0
spellingShingle Original Article
Masse, M
Glippa, V
Saad, H
Le Bloas, R
Gauffeny, I
Berthou, C
Czjzek, M
Cormier, P
Cosson, B
An eIF4E-interacting peptide induces cell death in cancer cell lines
title An eIF4E-interacting peptide induces cell death in cancer cell lines
title_full An eIF4E-interacting peptide induces cell death in cancer cell lines
title_fullStr An eIF4E-interacting peptide induces cell death in cancer cell lines
title_full_unstemmed An eIF4E-interacting peptide induces cell death in cancer cell lines
title_short An eIF4E-interacting peptide induces cell death in cancer cell lines
title_sort eif4e-interacting peptide induces cell death in cancer cell lines
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4237268/
https://www.ncbi.nlm.nih.gov/pubmed/25356869
http://dx.doi.org/10.1038/cddis.2014.457
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