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Streamlining DNA Barcoding Protocols: Automated DNA Extraction and a New cox1 Primer in Arachnid Systematics

BACKGROUND: DNA barcoding is a popular tool in taxonomic and phylogenetic studies, but for most animal lineages protocols for obtaining the barcoding sequences—mitochondrial cytochrome C oxidase subunit I (cox1 AKA CO1)—are not standardized. Our aim was to explore an optimal strategy for arachnids,...

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Detalles Bibliográficos
Autores principales: Vidergar, Nina, Toplak, Nataša, Kuntner, Matjaž
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4240537/
https://www.ncbi.nlm.nih.gov/pubmed/25415202
http://dx.doi.org/10.1371/journal.pone.0113030