Enzymatic generation of peptides flanked by basic amino acids to obtain MS/MS spectra with 2× sequence coverage

RATIONALE: Tandem mass (MS/MS) spectra generated by collision-induced dissociation (CID) typically lack redundant peptide sequence information in the form of e.g. b- and y-ion series due to frequent use of sequence-specific endopeptidases cleaving C- or N-terminal to Arg or Lys residues. METHODS: He...

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Detalles Bibliográficos
Autores principales: Ebhardt, H Alexander, Nan, Jie, Chaulk, Steven G, Fahlman, Richard P, Aebersold, Ruedi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BlackWell Publishing Ltd 2014
Materias:
Research Articles
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4241039/
https://www.ncbi.nlm.nih.gov/pubmed/25380496
http://dx.doi.org/10.1002/rcm.7069

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4241039/
https://www.ncbi.nlm.nih.gov/pubmed/25380496
http://dx.doi.org/10.1002/rcm.7069

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