Heterogeneous Red Blood Cell Adhesion and Deformability in Sickle Cell Disease

We present a microfluidic approach that allows simultaneous interrogation of RBC properties in physiological flow conditions at a single cell level. With this method, we studied healthy hemoglobin A (HbA) and homozygous sickle hemoglobin (HbS) containing RBCs using whole blood samples from twelve su...

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Detalles Bibliográficos
Autores principales: Alapan, Yunus, Little, Jane A., Gurkan, Umut A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2014
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4241514/
https://www.ncbi.nlm.nih.gov/pubmed/25417696
http://dx.doi.org/10.1038/srep07173
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author Alapan, Yunus
Little, Jane A.
Gurkan, Umut A.
author_facet Alapan, Yunus
Little, Jane A.
Gurkan, Umut A.
author_sort Alapan, Yunus
collection PubMed
description We present a microfluidic approach that allows simultaneous interrogation of RBC properties in physiological flow conditions at a single cell level. With this method, we studied healthy hemoglobin A (HbA) and homozygous sickle hemoglobin (HbS) containing RBCs using whole blood samples from twelve subjects. We report that HbS-containing RBCs are heterogeneous in terms of adhesion and deformability in flow.
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spelling pubmed-42415142014-11-25 Heterogeneous Red Blood Cell Adhesion and Deformability in Sickle Cell Disease Alapan, Yunus Little, Jane A. Gurkan, Umut A. Sci Rep Article We present a microfluidic approach that allows simultaneous interrogation of RBC properties in physiological flow conditions at a single cell level. With this method, we studied healthy hemoglobin A (HbA) and homozygous sickle hemoglobin (HbS) containing RBCs using whole blood samples from twelve subjects. We report that HbS-containing RBCs are heterogeneous in terms of adhesion and deformability in flow. Nature Publishing Group 2014-11-24 /pmc/articles/PMC4241514/ /pubmed/25417696 http://dx.doi.org/10.1038/srep07173 Text en Copyright © 2014, Macmillan Publishers Limited. All rights reserved http://creativecommons.org/licenses/by-nc-nd/4.0/ This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder in order to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/4.0/
spellingShingle Article
Alapan, Yunus
Little, Jane A.
Gurkan, Umut A.
Heterogeneous Red Blood Cell Adhesion and Deformability in Sickle Cell Disease
title Heterogeneous Red Blood Cell Adhesion and Deformability in Sickle Cell Disease
title_full Heterogeneous Red Blood Cell Adhesion and Deformability in Sickle Cell Disease
title_fullStr Heterogeneous Red Blood Cell Adhesion and Deformability in Sickle Cell Disease
title_full_unstemmed Heterogeneous Red Blood Cell Adhesion and Deformability in Sickle Cell Disease
title_short Heterogeneous Red Blood Cell Adhesion and Deformability in Sickle Cell Disease
title_sort heterogeneous red blood cell adhesion and deformability in sickle cell disease
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4241514/
https://www.ncbi.nlm.nih.gov/pubmed/25417696
http://dx.doi.org/10.1038/srep07173
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