Synonymous Rare Arginine Codons and tRNA Abundance Affect Protein Production and Quality of TEV Protease Variant

It has been identified that a TEV protease (TEVp) variant, TEVp(5M), displays improved solubility. Here, we constructed fifteen TEVp(5M) variants with one or more of six rare arginine codons in the coding sequence replaced with abundant E. coli arginine codons. These codon variants expressed in eith...

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Detalles Bibliográficos
Autores principales: Fang, Jie, Zou, Lingling, Zhou, Xuan, Cheng, Beijiu, Fan, Jun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2014
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4245098/
https://www.ncbi.nlm.nih.gov/pubmed/25426854
http://dx.doi.org/10.1371/journal.pone.0112254

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4245098/
https://www.ncbi.nlm.nih.gov/pubmed/25426854
http://dx.doi.org/10.1371/journal.pone.0112254

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