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Site-Specific Labeling of Neurotrophins and Their Receptors via Short and Versatile Peptide Tags

We present a toolbox for the study of molecular interactions occurring between NGF and its receptors. By means of a suitable insertional mutagenesis method we show the insertion of an 8 amino acid tag (A4) into the sequence of NGF and of 12 amino acid tags (A1 and S6) into the sequence of TrkA and P...

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Detalles Bibliográficos
Autores principales: Marchetti, Laura, De Nadai, Teresa, Bonsignore, Fulvio, Calvello, Mariantonietta, Signore, Giovanni, Viegi, Alessandro, Beltram, Fabio, Luin, Stefano, Cattaneo, Antonino
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4245215/
https://www.ncbi.nlm.nih.gov/pubmed/25426999
http://dx.doi.org/10.1371/journal.pone.0113708