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Site-Specific Labeling of Neurotrophins and Their Receptors via Short and Versatile Peptide Tags
We present a toolbox for the study of molecular interactions occurring between NGF and its receptors. By means of a suitable insertional mutagenesis method we show the insertion of an 8 amino acid tag (A4) into the sequence of NGF and of 12 amino acid tags (A1 and S6) into the sequence of TrkA and P...
Autores principales: | Marchetti, Laura, De Nadai, Teresa, Bonsignore, Fulvio, Calvello, Mariantonietta, Signore, Giovanni, Viegi, Alessandro, Beltram, Fabio, Luin, Stefano, Cattaneo, Antonino |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4245215/ https://www.ncbi.nlm.nih.gov/pubmed/25426999 http://dx.doi.org/10.1371/journal.pone.0113708 |
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