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UV-induced Spectral Shift and Protonation of DNA Fluorescent Dye Hoechst 33258
DNA-bound Hoechst 33258 is readily excited with UV light and emits blue fluorescence, however, upon exposure to UV, the dye undergoes photobleaching as well as photoconversion to a blue-excited green-emitting form. We demonstrate that the UV-generated green-emitting form of Hoechst 33258 exhibits sp...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer US
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4246133/ https://www.ncbi.nlm.nih.gov/pubmed/25312832 http://dx.doi.org/10.1007/s10895-014-1468-y |