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A DNA-based molecular probe for optically reporting cellular traction forces
We developed molecular tension probes (TPs) that report traction forces of adherent cells with high spatial resolution, can be linked to virtually any surface, and obviate monitoring deformations of elastic substrates. TPs consist of DNA hairpins conjugated to fluorophore-quencher pairs that unfold...
| Autores principales: | , , , , , , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
2014
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4247985/ https://www.ncbi.nlm.nih.gov/pubmed/25306545 http://dx.doi.org/10.1038/nmeth.3145 |
| _version_ | 1782346738511642624 |
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| author | Blakely, Brandon L. Dumelin, Christoph E. Trappmann, Britta McGregor, Lynn M. Choi, Colin K. Anthony, Peter C. Duesterberg, Van K. Baker, Brendon M. Block, Steven M. Liu, David R. Chen, Christopher S. |
| author_facet | Blakely, Brandon L. Dumelin, Christoph E. Trappmann, Britta McGregor, Lynn M. Choi, Colin K. Anthony, Peter C. Duesterberg, Van K. Baker, Brendon M. Block, Steven M. Liu, David R. Chen, Christopher S. |
| author_sort | Blakely, Brandon L. |
| collection | PubMed |
| description | We developed molecular tension probes (TPs) that report traction forces of adherent cells with high spatial resolution, can be linked to virtually any surface, and obviate monitoring deformations of elastic substrates. TPs consist of DNA hairpins conjugated to fluorophore-quencher pairs that unfold and fluoresce when subjected to specific forces. We applied TPs to reveal that cellular traction forces are heterogeneous within focal adhesions and localized at their distal edges. |
| format | Online Article Text |
| id | pubmed-4247985 |
| institution | National Center for Biotechnology Information |
| language | English |
| publishDate | 2014 |
| record_format | MEDLINE/PubMed |
| spelling | pubmed-42479852015-06-01 A DNA-based molecular probe for optically reporting cellular traction forces Blakely, Brandon L. Dumelin, Christoph E. Trappmann, Britta McGregor, Lynn M. Choi, Colin K. Anthony, Peter C. Duesterberg, Van K. Baker, Brendon M. Block, Steven M. Liu, David R. Chen, Christopher S. Nat Methods Article We developed molecular tension probes (TPs) that report traction forces of adherent cells with high spatial resolution, can be linked to virtually any surface, and obviate monitoring deformations of elastic substrates. TPs consist of DNA hairpins conjugated to fluorophore-quencher pairs that unfold and fluoresce when subjected to specific forces. We applied TPs to reveal that cellular traction forces are heterogeneous within focal adhesions and localized at their distal edges. 2014-10-12 2014-12 /pmc/articles/PMC4247985/ /pubmed/25306545 http://dx.doi.org/10.1038/nmeth.3145 Text en http://www.nature.com/authors/editorial_policies/license.html#terms Users may view, print, copy, and download text and data-mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use:http://www.nature.com/authors/editorial_policies/license.html#terms |
| spellingShingle | Article Blakely, Brandon L. Dumelin, Christoph E. Trappmann, Britta McGregor, Lynn M. Choi, Colin K. Anthony, Peter C. Duesterberg, Van K. Baker, Brendon M. Block, Steven M. Liu, David R. Chen, Christopher S. A DNA-based molecular probe for optically reporting cellular traction forces |
| title | A DNA-based molecular probe for optically reporting cellular traction forces |
| title_full | A DNA-based molecular probe for optically reporting cellular traction forces |
| title_fullStr | A DNA-based molecular probe for optically reporting cellular traction forces |
| title_full_unstemmed | A DNA-based molecular probe for optically reporting cellular traction forces |
| title_short | A DNA-based molecular probe for optically reporting cellular traction forces |
| title_sort | dna-based molecular probe for optically reporting cellular traction forces |
| topic | Article |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4247985/ https://www.ncbi.nlm.nih.gov/pubmed/25306545 http://dx.doi.org/10.1038/nmeth.3145 |
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