Differential spatial and structural organization of the X chromosome underlies dosage compensation in C. elegans

The adjustment of X-linked gene expression to the X chromosome copy number (dosage compensation [DC]) has been widely studied as a model of chromosome-wide gene regulation. In Caenorhabditis elegans, DC is achieved by twofold down-regulation of gene expression from both Xs in hermaphrodites. We show...

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Autores principales: Sharma, Rahul, Jost, Daniel, Kind, Jop, Gómez-Saldivar, Georgina, van Steensel, Bas, Askjaer, Peter, Vaillant, Cédric, Meister, Peter
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cold Spring Harbor Laboratory Press 2014
Materias:
Research Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4248290/
https://www.ncbi.nlm.nih.gov/pubmed/25452271
http://dx.doi.org/10.1101/gad.248864.114
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author Sharma, Rahul
Jost, Daniel
Kind, Jop
Gómez-Saldivar, Georgina
van Steensel, Bas
Askjaer, Peter
Vaillant, Cédric
Meister, Peter
author_facet Sharma, Rahul
Jost, Daniel
Kind, Jop
Gómez-Saldivar, Georgina
van Steensel, Bas
Askjaer, Peter
Vaillant, Cédric
Meister, Peter
author_sort Sharma, Rahul
collection PubMed
description The adjustment of X-linked gene expression to the X chromosome copy number (dosage compensation [DC]) has been widely studied as a model of chromosome-wide gene regulation. In Caenorhabditis elegans, DC is achieved by twofold down-regulation of gene expression from both Xs in hermaphrodites. We show that in males, the single X chromosome interacts with nuclear pore proteins, while in hermaphrodites, the DC complex (DCC) impairs this interaction and alters X localization. Our results put forward a structural model of DC in which X-specific sequences locate the X chromosome in transcriptionally active domains in males, while the DCC prevents this in hermaphrodites.
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spelling pubmed-42482902015-06-01 Differential spatial and structural organization of the X chromosome underlies dosage compensation in C. elegans Sharma, Rahul Jost, Daniel Kind, Jop Gómez-Saldivar, Georgina van Steensel, Bas Askjaer, Peter Vaillant, Cédric Meister, Peter Genes Dev Research Communication The adjustment of X-linked gene expression to the X chromosome copy number (dosage compensation [DC]) has been widely studied as a model of chromosome-wide gene regulation. In Caenorhabditis elegans, DC is achieved by twofold down-regulation of gene expression from both Xs in hermaphrodites. We show that in males, the single X chromosome interacts with nuclear pore proteins, while in hermaphrodites, the DC complex (DCC) impairs this interaction and alters X localization. Our results put forward a structural model of DC in which X-specific sequences locate the X chromosome in transcriptionally active domains in males, while the DCC prevents this in hermaphrodites. Cold Spring Harbor Laboratory Press 2014-12-01 /pmc/articles/PMC4248290/ /pubmed/25452271 http://dx.doi.org/10.1101/gad.248864.114 Text en © 2014 Sharma et al.; Published by Cold Spring Harbor Laboratory Press http://creativecommons.org/licenses/by-nc/4.0/ This article is distributed exclusively by Cold Spring Harbor Laboratory Press for the first six months after the full-issue publication date (see http://genesdev.cshlp.org/site/misc/terms.xhtml). After six months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.
spellingShingle Research Communication
Sharma, Rahul
Jost, Daniel
Kind, Jop
Gómez-Saldivar, Georgina
van Steensel, Bas
Askjaer, Peter
Vaillant, Cédric
Meister, Peter
Differential spatial and structural organization of the X chromosome underlies dosage compensation in C. elegans
title Differential spatial and structural organization of the X chromosome underlies dosage compensation in C. elegans
title_full Differential spatial and structural organization of the X chromosome underlies dosage compensation in C. elegans
title_fullStr Differential spatial and structural organization of the X chromosome underlies dosage compensation in C. elegans
title_full_unstemmed Differential spatial and structural organization of the X chromosome underlies dosage compensation in C. elegans
title_short Differential spatial and structural organization of the X chromosome underlies dosage compensation in C. elegans
title_sort differential spatial and structural organization of the x chromosome underlies dosage compensation in c. elegans
topic Research Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4248290/
https://www.ncbi.nlm.nih.gov/pubmed/25452271
http://dx.doi.org/10.1101/gad.248864.114
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