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Reduction of Photo Bleaching and Long Term Archiving of Chemically Cleared GFP-Expressing Mouse Brains

Tissue clearing allows microscopy of large specimens as whole mouse brains or embryos. However, lipophilic tissue clearing agents as dibenzyl ether limit storage time of GFP-expressing samples to several days and do not prevent them from photobleaching during microscopy. To preserve GFP fluorescence...

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Detalles Bibliográficos
Autores principales: Becker, Klaus, Hahn, Christian Markus, Saghafi, Saiedeh, Jährling, Nina, Wanis, Martina, Dodt, Hans-Ulrich
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4252090/
https://www.ncbi.nlm.nih.gov/pubmed/25463047
http://dx.doi.org/10.1371/journal.pone.0114149