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Reduction of Photo Bleaching and Long Term Archiving of Chemically Cleared GFP-Expressing Mouse Brains
Tissue clearing allows microscopy of large specimens as whole mouse brains or embryos. However, lipophilic tissue clearing agents as dibenzyl ether limit storage time of GFP-expressing samples to several days and do not prevent them from photobleaching during microscopy. To preserve GFP fluorescence...
Autores principales: | Becker, Klaus, Hahn, Christian Markus, Saghafi, Saiedeh, Jährling, Nina, Wanis, Martina, Dodt, Hans-Ulrich |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4252090/ https://www.ncbi.nlm.nih.gov/pubmed/25463047 http://dx.doi.org/10.1371/journal.pone.0114149 |
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