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Construction of a mariner-based transposon vector for use in insertion sequence mutagenesis in selected members of the Rhizobiaceae

BACKGROUND: The Rhizobiaceae family of Gram-negative bacteria often engage in symbiosis with plants of economic importance. Historically, genetic studies to identify the function of individual genes, and characterize the biology of these bacteria have relied on the use of classical transposon mutage...

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Detalles Bibliográficos
Autores principales: Perry, Benjamin J, Yost, Christopher K
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4255674/
https://www.ncbi.nlm.nih.gov/pubmed/25433486
http://dx.doi.org/10.1186/s12866-014-0298-z