Cargando…
Paraquat Induces Apoptosis through Cytochrome C Release and ERK Activation
Paraquat has been suggested to induce apoptosis by generation of reactive oxygen species (ROS). However, little is known about the mechanism of paraquat-induced apoptosis. Here, we demonstrate that extracellular signal-regulated protein kinase (ERK) is required for paraquat-induced apoptosis in NIH3...
Autores principales: | , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Korean Society of Applied Pharmacology
2014
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4256029/ https://www.ncbi.nlm.nih.gov/pubmed/25489417 http://dx.doi.org/10.4062/biomolther.2014.115 |
_version_ | 1782347527105806336 |
---|---|
author | Seo, Hong Joo Choi, Sang Joon Lee, Jung-Hee |
author_facet | Seo, Hong Joo Choi, Sang Joon Lee, Jung-Hee |
author_sort | Seo, Hong Joo |
collection | PubMed |
description | Paraquat has been suggested to induce apoptosis by generation of reactive oxygen species (ROS). However, little is known about the mechanism of paraquat-induced apoptosis. Here, we demonstrate that extracellular signal-regulated protein kinase (ERK) is required for paraquat-induced apoptosis in NIH3T3 cells. Paraquat treatment resulted in activation of ERK, and U0126, inhibitors of the MEK/ERK signaling pathway, prevented apoptosis. Moreover, paraquat-induced apoptosis was associated with cytochrome C release, which could be prevented by treatment with the MEK inhibitors. Taken together, our findings suggest that ERK activation plays an active role in mediating paraquat-induced apoptosis of NIH3T3 cells. |
format | Online Article Text |
id | pubmed-4256029 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | The Korean Society of Applied Pharmacology |
record_format | MEDLINE/PubMed |
spelling | pubmed-42560292014-12-08 Paraquat Induces Apoptosis through Cytochrome C Release and ERK Activation Seo, Hong Joo Choi, Sang Joon Lee, Jung-Hee Biomol Ther (Seoul) Original Article Paraquat has been suggested to induce apoptosis by generation of reactive oxygen species (ROS). However, little is known about the mechanism of paraquat-induced apoptosis. Here, we demonstrate that extracellular signal-regulated protein kinase (ERK) is required for paraquat-induced apoptosis in NIH3T3 cells. Paraquat treatment resulted in activation of ERK, and U0126, inhibitors of the MEK/ERK signaling pathway, prevented apoptosis. Moreover, paraquat-induced apoptosis was associated with cytochrome C release, which could be prevented by treatment with the MEK inhibitors. Taken together, our findings suggest that ERK activation plays an active role in mediating paraquat-induced apoptosis of NIH3T3 cells. The Korean Society of Applied Pharmacology 2014-11 2014-11-30 /pmc/articles/PMC4256029/ /pubmed/25489417 http://dx.doi.org/10.4062/biomolther.2014.115 Text en Copyright ©2014, The Korean Society of Applied Pharmacology http://creativecommons.org/licenses/by-nc/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Article Seo, Hong Joo Choi, Sang Joon Lee, Jung-Hee Paraquat Induces Apoptosis through Cytochrome C Release and ERK Activation |
title | Paraquat Induces Apoptosis through Cytochrome C Release and ERK Activation |
title_full | Paraquat Induces Apoptosis through Cytochrome C Release and ERK Activation |
title_fullStr | Paraquat Induces Apoptosis through Cytochrome C Release and ERK Activation |
title_full_unstemmed | Paraquat Induces Apoptosis through Cytochrome C Release and ERK Activation |
title_short | Paraquat Induces Apoptosis through Cytochrome C Release and ERK Activation |
title_sort | paraquat induces apoptosis through cytochrome c release and erk activation |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4256029/ https://www.ncbi.nlm.nih.gov/pubmed/25489417 http://dx.doi.org/10.4062/biomolther.2014.115 |
work_keys_str_mv | AT seohongjoo paraquatinducesapoptosisthroughcytochromecreleaseanderkactivation AT choisangjoon paraquatinducesapoptosisthroughcytochromecreleaseanderkactivation AT leejunghee paraquatinducesapoptosisthroughcytochromecreleaseanderkactivation |