Cargando…

Direct analysis of mAb aggregates in mammalian cell culture supernatant

BACKGROUND: Protein aggregation during monoclonal antibody (mAb) production can occur in upstream and downstream processing (DSP). Current methods to determine aggregate formation during cell culture include size exclusion chromatography (SEC) with a previous affinity chromatography step in order to...

Descripción completa

Detalles Bibliográficos
Autores principales:	Paul, Albert J, Schwab, Karen, Hesse, Friedemann
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	BioMed Central 2014
Materias:	Methodology Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4256052/ https://www.ncbi.nlm.nih.gov/pubmed/25431119 http://dx.doi.org/10.1186/s12896-014-0099-3

Ejemplares similares

Characterization of mAb aggregates in a mammalian cell culture production process
por: Paul, Albert, et al.
Publicado: (2013)

SEPPA-mAb: spatial epitope prediction of protein antigens for mAbs
por: Qiu, Tianyi, et al.
Publicado: (2023)

A new approach to quantification of mAb aggregates using peptide affinity probes
por: Cheung, Crystal S. F., et al.
Publicado: (2017)

Protective mAbs and Cross-Reactive mAbs Raised by Immunization with Engineered Marburg Virus GPs
por: Fusco, Marnie L., et al.
Publicado: (2015)

Evaluation of fluorescent dyes to measure protein aggregation within mammalian cell culture supernatants
por: Oshinbolu, Sheun, et al.
Publicado: (2018)

The Different Colors of mAbs in Solution
por: Ambrogelly, Alexandre
Publicado: (2021)

Correction: Protective mAbs and Cross-Reactive mAbs Raised by Immunization with Engineered Marburg Virus GPs
por: Fusco, Marnie L., et al.
Publicado: (2015)

Understanding the Stabilizing Effect of Histidine on mAb Aggregation: A Molecular Dynamics Study
por: Saurabh, Suman, et al.
Publicado: (2022)

Cellular tools for biosimilar mAb analysis
por: Lindemann, Carsten, et al.
Publicado: (2013)

Stability enhancement in a mAb and Fab coformulation
por: Zhang, Hongyu, et al.
Publicado: (2020)

The impact of forced degradation conditions on mAb dimer formation and subsequent influence on aggregation propensity
por: Knight, Michael J., et al.
Publicado: (2022)

Evolving trends in mAb production processes
por: Shukla, Abhinav A., et al.
Publicado: (2017)

State of the art in anti-cancer mAbs
por: Chiavenna, S. M., et al.
Publicado: (2017)

Identification of process parameters influencing product quality in mammalian cell culture
por: Paul, Albert J, et al.
Publicado: (2015)

Simultaneous assessment of cytotoxic T lymphocyte responses against multiple viral infections by combined usage of optimal epitope matrices, anti- CD3 mAb T-cell expansion and "RecycleSpot"
por: Bihl, Florian K, et al.
Publicado: (2005)

2D fluorescence spectroscopy for real-time aggregation monitoring in upstream processing
por: Schwab, Karen, et al.
Publicado: (2013)

Host cell protein platform assay development for therapeutic mAb bioprocessing using mammalian cells
por: Kochanowski, Nadine, et al.
Publicado: (2015)

Estimating Extrinsic Dyes for Fluorometric Online Monitoring of Antibody Aggregation in CHO Fed-Batch Cultivations
por: Schwab, Karen, et al.
Publicado: (2017)

Pan-sarbecovirus mAb protects from variants
por: Flemming, Alexandra
Publicado: (2021)

A mAb for the detection of the antiretroviral drug emtricitabine
por: Youngpairoj, Ae S., et al.
Publicado: (2022)

Subunit selectivity and epitope characterization of mAbs directed against the GABAA/benzodiazepine receptor
Publicado: (1990)

Will a mAb-Based Immunotherapy Directed against Cancer Stem Cells Be Feasible?
por: Santamaria, Silvia, et al.
Publicado: (2017)

Novel strategy for a high-yielding mAb-producing CHO strain (overexpression of non-coding RNA enhanced proliferation and improved mAb yield)
por: Tabuchi, Hisahiro
Publicado: (2013)

Anti-GD2 mAb and Vorinostat synergize in the treatment of neuroblastoma
por: Kroesen, Michiel, et al.
Publicado: (2016)

The effect of mAb and excipient cryoconcentration on long-term frozen storage stability – part 2: Aggregate formation and oxidation
por: Bluemel, Oliver, et al.
Publicado: (2021)

Purification of native histidine-rich protein 2 (nHRP2) from Plasmodium falciparum culture supernatant, infected RBCs, and parasite lysate
por: Singh, Balwan, et al.
Publicado: (2021)

Discovery of cell surface vimentin targeting mAb for direct disruption of GBM tumor initiating cells
por: Noh, Hyangsoon, et al.
Publicado: (2016)

Conservancy of mAb Epitopes in Ebolavirus Glycoproteins of Previous and 2014 Outbreaks
por: Ponomarenko, Julia, et al.
Publicado: (2014)

Developmental changes in heparan sulfate expression: in situ detection with mAbs
Publicado: (1992)

Self-Interaction Chromatography of mAbs: Accurate Measurement of Dead Volumes
por: Hedberg, S. H. M., et al.
Publicado: (2015)

Germinality does not necessarily define mAb expression and thermal stability
por: Schwaigerlehner, Linda, et al.
Publicado: (2019)

The Role of Complement in the Mechanism of Action of Therapeutic Anti-Cancer mAbs
por: Golay, Josée, et al.
Publicado: (2020)

Endoplasmic reticulum retention motif fused to recombinant anti-cancer monoclonal antibody (mAb) CO17-1A affects mAb expression and plant stress response
por: Song, Ilchan, et al.
Publicado: (2018)

Characterization of Two Novel mAbs Recognizing Different Epitopes on CD43
por: Kim, Soseul, et al.
Publicado: (2014)

Strategies for adaptation of mAb-producing CHO cells to serum-free medium
por: Costa, A Rita, et al.
Publicado: (2011)

Successful radioimmunotherapy of established syngeneic rat colon carcinoma with (211)At-mAb
por: Eriksson, Sophie E, et al.
Publicado: (2013)

Deamidated gluten: specific detection and characterisation with INRA-DG1 mAb
por: Tranquet, Olivier, et al.
Publicado: (2015)

Glycosylation characterization of therapeutic mAbs by top- and middle-down mass spectrometry
por: Tran, Bao Quoc, et al.
Publicado: (2015)

Plant Recycling for Molecular Biofarming to Produce Recombinant Anti-Cancer mAb
por: Kim, Deuk-Su, et al.
Publicado: (2016)

Tanezumab: a selective humanized mAb for chronic lower back pain
por: Webb, Michael P, et al.
Publicado: (2018)

Cannot write session to /tmp/vufind_sessions/sess_pdmfkb6mpd4nvv5qqdo0q6u84n