A “Proteomic Ruler” for Protein Copy Number and Concentration Estimation without Spike-in Standards

Absolute protein quantification using mass spectrometry (MS)-based proteomics delivers protein concentrations or copy numbers per cell. Existing methodologies typically require a combination of isotope-labeled spike-in references, cell counting, and protein concentration measurements. Here we presen...

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Detalles Bibliográficos
Autores principales: Wiśniewski, Jacek R., Hein, Marco Y., Cox, Jürgen, Mann, Matthias
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The American Society for Biochemistry and Molecular Biology 2014
Materias:
Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4256500/
https://www.ncbi.nlm.nih.gov/pubmed/25225357
http://dx.doi.org/10.1074/mcp.M113.037309

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4256500/
https://www.ncbi.nlm.nih.gov/pubmed/25225357
http://dx.doi.org/10.1074/mcp.M113.037309

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