Increased Expression of AQP 1 and AQP 5 in Rat Lungs Ventilated with Low Tidal Volume is Time Dependent
BACKGROUND AND GOALS: Mechanical ventilation (MV) can induce or worsen pulmonary oedema. Aquaporins (AQPs) facilitate the selective and rapid bi-directional movement of water. Their role in the development and resolution of pulmonary oedema is controversial. Our objectives are to determine if prolon...
Autores principales: | , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2014
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4260863/ https://www.ncbi.nlm.nih.gov/pubmed/25489856 http://dx.doi.org/10.1371/journal.pone.0114247 |
_version_ | 1782348237410140160 |
---|---|
author | Fabregat, Gustavo García-de-la-Asunción, José Sarriá, Benjamín Cortijo, Julio De Andrés, José Mata, Manuel Pastor, Ernesto Belda, Francisco Javier |
author_facet | Fabregat, Gustavo García-de-la-Asunción, José Sarriá, Benjamín Cortijo, Julio De Andrés, José Mata, Manuel Pastor, Ernesto Belda, Francisco Javier |
author_sort | Fabregat, Gustavo |
collection | PubMed |
description | BACKGROUND AND GOALS: Mechanical ventilation (MV) can induce or worsen pulmonary oedema. Aquaporins (AQPs) facilitate the selective and rapid bi-directional movement of water. Their role in the development and resolution of pulmonary oedema is controversial. Our objectives are to determine if prolonged MV causes lung oedema and changes in the expression of AQP 1 and AQP 5 in rats. METHODS: 25 male Wistar rats were subjected to MV with a tidal volume of 10 ml/kg, during 2 hours (n = 12) and 4 hours (n = 13). Degree of oedema was compared with a group of non-ventilated rats (n = 5). The expression of AQP 1 and AQP 5 were determined by western immunoblotting, measuring the amount of mRNA (previously amplified by RT-PCR) and immunohistochemical staining of AQPs 1 and 5 in lung samples from all groups. RESULTS: Lung oedema and alveolar-capillary membrane permeability did not change during MV. AQP-5 steady state levels in the western blot were increased (p<0.01) at 2 h and 4 h of MV. But in AQP-1 expression these differences were not found. However, the amount of mRNA for AQP-1 was increased at 2 h and 4 h of MV; and for AQP 5 at 4 h of MV. These findings were corroborated by representative immunohistochemical lung samples. CONCLUSION: In lungs from rats ventilated with a low tidal volume the expression of AQP 5 increases gradually with MV duration, but does not cause pulmonary oedema or changes in lung permeability. AQPs may have a protective effect against the oedema induced by MV. |
format | Online Article Text |
id | pubmed-4260863 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-42608632014-12-15 Increased Expression of AQP 1 and AQP 5 in Rat Lungs Ventilated with Low Tidal Volume is Time Dependent Fabregat, Gustavo García-de-la-Asunción, José Sarriá, Benjamín Cortijo, Julio De Andrés, José Mata, Manuel Pastor, Ernesto Belda, Francisco Javier PLoS One Research Article BACKGROUND AND GOALS: Mechanical ventilation (MV) can induce or worsen pulmonary oedema. Aquaporins (AQPs) facilitate the selective and rapid bi-directional movement of water. Their role in the development and resolution of pulmonary oedema is controversial. Our objectives are to determine if prolonged MV causes lung oedema and changes in the expression of AQP 1 and AQP 5 in rats. METHODS: 25 male Wistar rats were subjected to MV with a tidal volume of 10 ml/kg, during 2 hours (n = 12) and 4 hours (n = 13). Degree of oedema was compared with a group of non-ventilated rats (n = 5). The expression of AQP 1 and AQP 5 were determined by western immunoblotting, measuring the amount of mRNA (previously amplified by RT-PCR) and immunohistochemical staining of AQPs 1 and 5 in lung samples from all groups. RESULTS: Lung oedema and alveolar-capillary membrane permeability did not change during MV. AQP-5 steady state levels in the western blot were increased (p<0.01) at 2 h and 4 h of MV. But in AQP-1 expression these differences were not found. However, the amount of mRNA for AQP-1 was increased at 2 h and 4 h of MV; and for AQP 5 at 4 h of MV. These findings were corroborated by representative immunohistochemical lung samples. CONCLUSION: In lungs from rats ventilated with a low tidal volume the expression of AQP 5 increases gradually with MV duration, but does not cause pulmonary oedema or changes in lung permeability. AQPs may have a protective effect against the oedema induced by MV. Public Library of Science 2014-12-09 /pmc/articles/PMC4260863/ /pubmed/25489856 http://dx.doi.org/10.1371/journal.pone.0114247 Text en © 2014 Fabregat et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Fabregat, Gustavo García-de-la-Asunción, José Sarriá, Benjamín Cortijo, Julio De Andrés, José Mata, Manuel Pastor, Ernesto Belda, Francisco Javier Increased Expression of AQP 1 and AQP 5 in Rat Lungs Ventilated with Low Tidal Volume is Time Dependent |
title | Increased Expression of AQP 1 and AQP 5 in Rat Lungs Ventilated with Low Tidal Volume is Time Dependent |
title_full | Increased Expression of AQP 1 and AQP 5 in Rat Lungs Ventilated with Low Tidal Volume is Time Dependent |
title_fullStr | Increased Expression of AQP 1 and AQP 5 in Rat Lungs Ventilated with Low Tidal Volume is Time Dependent |
title_full_unstemmed | Increased Expression of AQP 1 and AQP 5 in Rat Lungs Ventilated with Low Tidal Volume is Time Dependent |
title_short | Increased Expression of AQP 1 and AQP 5 in Rat Lungs Ventilated with Low Tidal Volume is Time Dependent |
title_sort | increased expression of aqp 1 and aqp 5 in rat lungs ventilated with low tidal volume is time dependent |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4260863/ https://www.ncbi.nlm.nih.gov/pubmed/25489856 http://dx.doi.org/10.1371/journal.pone.0114247 |
work_keys_str_mv | AT fabregatgustavo increasedexpressionofaqp1andaqp5inratlungsventilatedwithlowtidalvolumeistimedependent AT garciadelaasuncionjose increasedexpressionofaqp1andaqp5inratlungsventilatedwithlowtidalvolumeistimedependent AT sarriabenjamin increasedexpressionofaqp1andaqp5inratlungsventilatedwithlowtidalvolumeistimedependent AT cortijojulio increasedexpressionofaqp1andaqp5inratlungsventilatedwithlowtidalvolumeistimedependent AT deandresjose increasedexpressionofaqp1andaqp5inratlungsventilatedwithlowtidalvolumeistimedependent AT matamanuel increasedexpressionofaqp1andaqp5inratlungsventilatedwithlowtidalvolumeistimedependent AT pastorernesto increasedexpressionofaqp1andaqp5inratlungsventilatedwithlowtidalvolumeistimedependent AT beldafranciscojavier increasedexpressionofaqp1andaqp5inratlungsventilatedwithlowtidalvolumeistimedependent |