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α-Amylase inhibitory activity of some traditionally used medicinal species of Labiatae

BACKGROUND: Natural α-amylase inhibitors of herbal origin are an attractive therapeutic approach to control post-prandial hyperglycemia via reducing the glucose release from starch and delaying carbohydrate absorption. These compounds are able to inhibit the activity of the carbohydrate hydrolyzing...

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Autores principales: Safamansouri, Hedieh, Nikan, Marjan, Amin, Gholamreza, Sarkhail, Parisa, Gohari, Ahmad Reza, Kurepaz-Mahmoodabadi, Mahdieh, Saeidnia, Soodabeh
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4262240/
https://www.ncbi.nlm.nih.gov/pubmed/25506585
http://dx.doi.org/10.1186/s40200-014-0114-1
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author Safamansouri, Hedieh
Nikan, Marjan
Amin, Gholamreza
Sarkhail, Parisa
Gohari, Ahmad Reza
Kurepaz-Mahmoodabadi, Mahdieh
Saeidnia, Soodabeh
author_facet Safamansouri, Hedieh
Nikan, Marjan
Amin, Gholamreza
Sarkhail, Parisa
Gohari, Ahmad Reza
Kurepaz-Mahmoodabadi, Mahdieh
Saeidnia, Soodabeh
author_sort Safamansouri, Hedieh
collection PubMed
description BACKGROUND: Natural α-amylase inhibitors of herbal origin are an attractive therapeutic approach to control post-prandial hyperglycemia via reducing the glucose release from starch and delaying carbohydrate absorption. These compounds are able to inhibit the activity of the carbohydrate hydrolyzing enzymes in the small intestine and potentially useful in control of diabetes. The enlarged Lamiaceae (Labiatae) family contains about 6,900 to 7,200 species worldwide and many species of this family possess medicinal properties and have been used traditionally for treatment of chronic illnesses including diabetes. METHODS: In the present study particular species of Labiatae family from the genera, Phlomis, Satureja, Salvia, Scutellarua, Stachys and Hymenocrater, which are growing wildly in Iran, selected to evaluate for possible in vitro α-amylase inhibitory activity, compared to acarbose as a positive control. RESULTS: The inhibitory activities of all the herbal extracts were varied from 1.9 to 18.6 (IC50, μg/mL). Additionally, the ethyl acetate extract of P. bruguieri (IC50 = 1.9 μg/mL) and the butanol extract of P. persica (IC50 = 3.6 μg/mL) exhibited the lowest IC50 values among all the species as the most potent herbal extracts, while the inhibitory activity of S. sahendica and S. macrosiphon (ethyl acetate extracts) as well as P. caucasica (butanol extract) on α-amylase enzyme was observed as weak and did not reach at least to the 50% of the enzyme inhibition level. CONCLUSIONS: Taking together, P. bruguieri and P. persica among the Phlomis species can be the promising sources of α-amylase inhibitors. However, P. rigida, S. bizantina and H. bituminosus that exhibited moderate activity can be stand on second level of interest.
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spelling pubmed-42622402014-12-12 α-Amylase inhibitory activity of some traditionally used medicinal species of Labiatae Safamansouri, Hedieh Nikan, Marjan Amin, Gholamreza Sarkhail, Parisa Gohari, Ahmad Reza Kurepaz-Mahmoodabadi, Mahdieh Saeidnia, Soodabeh J Diabetes Metab Disord Research Article BACKGROUND: Natural α-amylase inhibitors of herbal origin are an attractive therapeutic approach to control post-prandial hyperglycemia via reducing the glucose release from starch and delaying carbohydrate absorption. These compounds are able to inhibit the activity of the carbohydrate hydrolyzing enzymes in the small intestine and potentially useful in control of diabetes. The enlarged Lamiaceae (Labiatae) family contains about 6,900 to 7,200 species worldwide and many species of this family possess medicinal properties and have been used traditionally for treatment of chronic illnesses including diabetes. METHODS: In the present study particular species of Labiatae family from the genera, Phlomis, Satureja, Salvia, Scutellarua, Stachys and Hymenocrater, which are growing wildly in Iran, selected to evaluate for possible in vitro α-amylase inhibitory activity, compared to acarbose as a positive control. RESULTS: The inhibitory activities of all the herbal extracts were varied from 1.9 to 18.6 (IC50, μg/mL). Additionally, the ethyl acetate extract of P. bruguieri (IC50 = 1.9 μg/mL) and the butanol extract of P. persica (IC50 = 3.6 μg/mL) exhibited the lowest IC50 values among all the species as the most potent herbal extracts, while the inhibitory activity of S. sahendica and S. macrosiphon (ethyl acetate extracts) as well as P. caucasica (butanol extract) on α-amylase enzyme was observed as weak and did not reach at least to the 50% of the enzyme inhibition level. CONCLUSIONS: Taking together, P. bruguieri and P. persica among the Phlomis species can be the promising sources of α-amylase inhibitors. However, P. rigida, S. bizantina and H. bituminosus that exhibited moderate activity can be stand on second level of interest. BioMed Central 2014-12-02 /pmc/articles/PMC4262240/ /pubmed/25506585 http://dx.doi.org/10.1186/s40200-014-0114-1 Text en © Safamansouri et al.; licensee BioMed Central. 2014 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Safamansouri, Hedieh
Nikan, Marjan
Amin, Gholamreza
Sarkhail, Parisa
Gohari, Ahmad Reza
Kurepaz-Mahmoodabadi, Mahdieh
Saeidnia, Soodabeh
α-Amylase inhibitory activity of some traditionally used medicinal species of Labiatae
title α-Amylase inhibitory activity of some traditionally used medicinal species of Labiatae
title_full α-Amylase inhibitory activity of some traditionally used medicinal species of Labiatae
title_fullStr α-Amylase inhibitory activity of some traditionally used medicinal species of Labiatae
title_full_unstemmed α-Amylase inhibitory activity of some traditionally used medicinal species of Labiatae
title_short α-Amylase inhibitory activity of some traditionally used medicinal species of Labiatae
title_sort α-amylase inhibitory activity of some traditionally used medicinal species of labiatae
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4262240/
https://www.ncbi.nlm.nih.gov/pubmed/25506585
http://dx.doi.org/10.1186/s40200-014-0114-1
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